超声造影评估大鼠单侧上尿路梗阻肾灌注改变  

Evaluation of renal perfusion in unilateral ureteral obstruction rats with contrast-enhanced ultrasounography

作　　者：杨芹[1] 么喜存[2] 孙浩然[3] 付胜伟[4] 郑翠敏[5] 

机构地区：[1]天津市滨海新区汉沽医院超声科,300480 [2]天津市滨海新区汉沽医院放射科,300480 [3]天津医科大学总医院放射科 [4]天津市滨海新区汉沽医院外科,300480 [5]天津市滨海新区汉沽医院急症科,300480

出　　处：《国际医学放射学杂志》2014年第3期205-208,共4页International Journal of Medical Radiology

基　　金：国家自然科学基金项目(81171316)

摘　　要：目的探讨超声造影(CEUS)技术及定量分析方法评估单侧输尿管梗阻(UUO)大鼠模型肾血流灌注改变的应用价值,为临床无创定量评价梗阻性肾病的血流灌注提供理论依据。方法 20只健康SD大鼠于麻醉下行左侧输尿管结扎术,建立UUO模型。分别于术前和术后7 d行CEUS检查,并于术后7 d CEUS检查后行相关病理检查。使用QLAB软件行双肾皮质血流灌注定量分析,并采用SPSS16.0统计软件分析时间-强度曲线(TIC)上升支斜率(A)、峰值强度(PI)、达峰时间(TTP)、受试者操作特征曲线下面积(AUC)。结果 UUO术后与术前比较,其TTP显著延长[(27.42±6.17)s∶(21.27±4.08)s,P<0.05],A[(0.88±0.79)dB/s∶(1.81±0.45)dB/s,P<0.05]及PI[(13.60±2.64)dB∶(16.50±1.36)dB,P<0.05]明显减低,AUC[(1 508.43±481.56)dB·s∶(1 309.40±306.93)dB·s,P<0.05]明显增高;健侧肾皮质TIC与术前比较,30 s之内相似,30 s后曲线下降缓慢,AUC明显增高[(1 311.50±406.14)dB·s∶(1 209.94±298.41)dB·s,P<0.05],A、PI和TTP无明显变化(P>0.05)。结论 CEUS及定量分析方法能反映UUO大鼠血流灌注状态的改变,为临床无创定量评价梗阻性肾病的血流灌注提供理论依据。Objective To explore renal blood perfusion characteristics in unilateral ureteral obstruction （UUO） rat with contrast-enhanced ultrasound （CEUS）, to provide a theoretical basis for clinical noninvasive quantitative evaluation of obstructive nephropathy. Methods Twenty Sprague Dawley rats were used in the study. CEUS were preformed before and 7 days after the UUO operation, and followed with histopathologic examination. Regional perfusion values of renal parenchyma were measured with QLAB software. SPSS16.0 statistical software was used for analasysing blood perfusion time to peak （TTP）, slope rate of ascending curve （A）, peak intensity （PI）,and area under ROC curve （AUC）. Results The perfusion values of hydronephrosis renal cortex were significantly changed in UUO rats compared to the preoperative values：TTP （27.42±6.17 s vs. 21.27±4.08s, P〈0.05）, A （0.88±0.79 dB/s vs. 1.81±0.45 dB/s, P〈0.05） and PI （13.60±2.64 dB vs. 16.50±1.36 dB, P〈0.05）, AUC （1 508.43±481.56 dB·s vs. 1 309.40±306.93） dB·s, P〈0.05）. The perfusion curve of the contralateral renal cortex was similar to the preoperative within the first 30s, however after 30s, the curve was slowed down significantly. The AUCs （1311.50 ±406.14 dB·s vs. 1209.94 ±298.41 dB·s, P〈0.05） were significantly increased. However, A, PI, and TTP had no significant difference between pre-operation and post-operation （P＆gt;0.05）. Conclusion CEUS and quantitative analysis techniques could reflect the change of kidney perfusion in UUO rats , it provides a theoretical basis for clinical noninvasive quantitative evaluation of obstructive nephropathnephropathy.

关 键 词：单侧输尿管梗阻 超声成像 血流灌注 对比增强 动物模型 

分 类 号：R693.2[医药卫生—泌尿科学]