牙鲆胚后发育过程中Ⅰ型脱碘酶基因的原位表达谱  

Expression pattern of Dio1 during the post-embryo development in Paralichthys olivaceus

作　　者：盖军伟[1] 鲍宝龙[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《上海海洋大学学报》2014年第3期321-328,共8页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31072201)

摘　　要：甲状腺激素T3在鱼类胚后发育与变态发育过程中发挥着重要作用,甲状腺激素T4通过Ⅰ型甲状腺氨酸脱碘酶(Dio1)生成T3,因此,调查Dio1基因在胚后发育包括变态发育过程中原位表达谱,有助于理解T3在胚后发育中的具体作用。利用RNA整体原位杂交技术,发现Dio1主要在牙鲆肠道、鳃、肝、肌肉、鳍、眼睛周围等器官和组织表达,鳃、肠道、奇鳍中的Dio1原位表达谱有一定的规律性,尤其在生骨肌细胞向鳍褶中迁移、臀鳍和背鳍(包括冠状幼鳍)的鳍条和支鳍骨的发生过程中,Dio1可能通过影响这些组织器官的T3水平而发挥重要作用。Thyroid hormone, mainly active triiodothyronine （T3）, plays important roles during the fish postembryo development including metamorphosis. Because of very small amount of T3 in whole body of larva, its distribution in different tissues during fish post-embryo development is not easy to be detected. As one kind of deiodinases, deiodinase Ⅰ can convert thyroxine （ T4 ） into the active T3. The expression pattern of Diol gene during post-embryo development can indirectly indicate the T3 distribution in different tissues. Diol gene is mainly expressed in intestine, gill, liver, body muscle, fin, and skin around eyes in post-embryo developing flounder Paralichthys olivaceus, indicating T3 regulates those tissues development. Specially during the process of anal fin and dorsal fin （ including crown-like larval fin） development, expression pattern of Diol was evidently associated with the formation of fin fold, fin rays and pterygiophores, suggesting thyroid hormone plays important role in regulating media fin development.

关 键 词：牙鲆 Ⅰ型脱碘酶 变态 冠状幼鳍 RNA整体原位杂交 

分 类 号：S917[农业科学—水产科学]