当归提取物配伍应用对小鼠结直肠癌的预防作用  

Preventive effect of combined administration of extracts from Danggui( Radix Angelicae Sinensis) on colorectum cancer in mice

作　　者：安静[1] 赵博琛[1] 吴清[1] 兰颐[1] 陈岩岩[1] 刘娜[1] 王文平[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2014年第5期309-313,I0002,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81073059);北京中医药大学创新团队发展计划项目(No.2011CXTD13);北京中医药大学自主课题项目(No.2013-JYBZZ-XS104)

摘　　要：目的研究当归多糖、当归油联合应用对诱发的小鼠炎症相关性结肠癌的化学预防作用,探讨二者联合应用的协同作用。方法建立模型对照组、当归油组、当归多糖低剂量组、当归多糖高剂量组、当归油+低剂量当归多糖组、当归油+高剂量当归多糖组及正常组,比较各组结直肠癌发病率,肿瘤大小、数目等。免疫组化法检测结直肠黏膜增殖细胞核抗原(PCNA)、环氧化酶-2(COX-2)、诱导型一氧化氮合成酶(iNOS)蛋白的表达水平。结果两种当归提取物均具有降低肿瘤发生率及抑制肿瘤发展的作用,以当归油与高剂量的当归多糖配伍对结直肠癌的发生发展抑制作用最强,与模型组相比(P<0.05)。各药物组PCNA、COX-2、iNOS蛋白表达明显低于模型对照组(P<0.05或P<0.001),当归油+高剂量当归多糖组、正常组表达较模型组差异最大(P<0.05)。结论当归多糖与当归油均能够对结直肠癌具有一定的防治作用;二者联合应用,具有一定的协同作用。二者防治癌症的作用可能与降低PCNA、COX-2、iNOS的表达有关。Objective To study the chemopreventive effect of combined administration of angelica polysaccharide （AP） and angelica oil （AO） from Danggui （Radix Angelicae Sinensis） on colorectum cancer in mice induced by azoxymethane/dextran sulfate sodium （AOM/DSS）. Methods All mice were divided into model group, AO group, low-dose AP group, high-dose AP group, AO ＋ low-dose AP group, AO ＋ high-dose AP group and blank group. The incidence, size and number of colorectum cancer, and expressions of colorectum PCNA, COX-2 and iNOS were compared among all groups. Results AP and AO all had effects of reducing the incidence of colorectum cancer and inhibiting tumor development, which was the most significant in AO ＋ high-dose AP group （compared with model group, P 〈 0.05 ）. The expressions of PCNA, COX-2 and iNOS decreased significantly in all drug groups （P 〈 0.05 or P 〈0.01 ）, which was the most significant in AO ＋ high-dose AP group （ compared with model group, P 〈 0.05）. Conclusion AP and AO all have preventive effect on colorectum cancer and their combined administration have a synergistic effect. The preventive effect of the combined administration of AP and AO on colorectum cancer may be related to they can reduce the expressions of PCNA, COX-2 and iNOS.

关 键 词：当归多糖 当归超临界提取物 结直肠癌 癌症预防 小鼠 

分 类 号：R285.5[医药卫生—中药学]