产β-甘露聚糖酶菌株的物理和化学诱变育种研究  

Physical and Chemical Mutagenesis Breeding of β-mannanase Strains

作　　者：李慧玲[1,2] 刘祖艳[1] 赵敏[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2014年第18期5741-5741,5750,共2页Journal of Anhui Agricultural Sciences

基　　金：黑龙江中医药大学优秀创新人才支持计划(2012RCQ13)

摘　　要：[目的]比较产β-甘露聚糖菌株的物理和化学诱变育种效果。[方法]以产β-甘露聚糖酶的Bacillus subtilis WD23为出发菌株，利用紫外线诱变和甲基磺酸乙酯诱变的方法提高酶的活性。[结果]紫外线照射时间选为20～30s，诱变后酶活提高了8．72％；1％的甲基磺矗乙酯诱变10min,酶活提高了1．29％。[结论]紫外线诱变效果好于甲基磺酸乙酸，该研究中，物理诱变效果好于化学诱变。[ Objective ] To compare physical and chemical mutagenesis breeding of 15-mannanase strains. [ Method]β-mannanase strains Bacillus subtilis WD23 was mutated by ultraviolet ray and ethylmethane sulfonate to increse its enzyme activity. [ Result] After mutated by UV irradiation for 20 -30 s or 1% thylmethane sulfonate for 10 min ,the enzyme activity of β-mannanase increase 8.72% and 1.29%, respectively. [ Conclusion ] The mutation effect by ultraviolet ray is better than ethylmethane sulfonate, and the physical mutation is better than chemical mutation.

关 键 词：Β-甘露聚糖酶 紫外线诱变 甲基磺酸乙酯诱变 

分 类 号：S182[农业科学—农业基础科学]