响应面法优化短梗五加中刺五加苷B和E的超声提取工艺  

Optimizing the Extraction Techniques of Eleutheroside B and Eleutheroside E from Eleutherococcus sessiliflorus Using Response Surface Methodology

作　　者：史伟国[1] 崔立勇[1,2] 佟庆[1,2] 袁寰[1] 吴莉莉[1] 周清波[1] 李洋[1] 

机构地区：[1]佳木斯大学,黑龙江佳木斯154007 [2]黑龙江省合江林业科学研究所,黑龙江佳木斯154002

出　　处：《安徽农业科学》2014年第18期5747-5748,5752,共3页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省森工总局科技项目(sgzjq201012);黑龙江省教育厅科学技术研究项目(12511577);佳木斯大学科学技术研究重点项目(Sz2011-013)

摘　　要：[目的]探索短梗五加中刺五加苷B、E的超声提取条件。[方法]在单因素试验的基础上,采用高效液相色谱法对短梗五加茎皮中的刺五加苷B和刺五加苷E的含量进行测定,并应用响应面法优化刺五加苷B、E的提取条件。[结果]刺五加苷B的最佳超声提取条件为:甲醇浓度为52.46%,超声提取时间75 min,料液比为40 g/ml;刺五加苷E的最佳提取工艺条件为:甲醇浓度49.36%,超声提取时间为75 min,料液比为40 g/ml。[结论]响应面法优化短梗五加刺五加苷B和E提取工艺合理可行,为提高刺五加苷B和E提取率提供了理论依据。[ Objective] This study aims to explore the best extraction techniques in elcutherosidc B, E ultrasonic from Eleutherococcus sessili- florus. [ Method] On the basis of single factor test, the content of eleutheroside B and E from Eleutherococcus sessiliflorus was determined by HPLC, and using response surface methodology, extraction conditions of eleutheroside B, E were optimized. [ Result] The best ultrasonic extraction conditions of eleutheroside B are as follows： methanol concentration 52.46% , ultrasonic extraction time 75 min, liquid to solid ratio 40 g/ml; the best uhtasonic extraction conditions of eleutberoside E are. methanol concentration 49.36% , ultrasonic extraction time 75 min, liquid to solid ratio of 40 g/ml. [ Conclusion] Response surface methodology is proper and practicable on the extraction of eleutheroside B and E from Eleutherococcus sessiliflorus, which will provide theoretical basis for improving the extraction rate.

关 键 词：短梗五加(Eleutherococcus sessiliflorus) 刺五加苷B 刺五加苷E 响应面法 

分 类 号：S567[农业科学—中草药栽培]