海藻酸钠纯度对微囊纤维化及囊内甲状旁腺组织功能的影响  

THE EFFECT OF ALGINATE PURITY ON FIBROSIS OF MICROCAPSULE AND THE FUNCTION OF MICROCAPSULATED PARATHYROID TISSUE

作　　者：刘克昌[1] 郭兰栓 张新江[1] 高占虎[1] 任太行 郭淑琴[2] 董健平[2] 

机构地区：[1]河北省保定市第一中心医院普通外科,保定071000 [2]河北省保定市第一中心医院内科

出　　处：《河北医科大学学报》2001年第2期83-85,共3页Journal of Hebei Medical University

摘　　要：目的研究海藻酸纯度对移植微囊及囊内人胚胎甲状旁腺组织功能的影响。方法将纯化及未纯化海藻酸微囊及微囊化人胚胎甲状旁腺组织分 4组异种移植于小鼠腹腔内 ,设立空囊对照组。测定人甲状旁腺素数值 ,通过测定具有特异性的人甲状旁腺素 ,以明确微囊内甲状旁腺组织的分泌功能 ,组织学检测微囊形态。结果移植后 2个月纯化组及未纯化组人甲状旁腺组织分泌功能有显著差异 (P <0 .0 5 ) ,分别为 (38.8± 13.7) pg/ml和(1.2± 0 .7) pg/ml,纯化及未纯化海藻酸钠制备空囊移植组 ,植入 1个月时微囊冲洗回收率分别为 (72± 18) %和(2 4± 13) % ,植入 2个月时为 (6 5± 2 1) %和 0。结论纯化的海藻酸制备微囊具有良好的生物相容性 。ObjectiveTo study the effect of alginate purity on implanted microcapsules and the function of human fetal parathyroid tissue in microcapsules.MethodsMicrocapsules and microencapsulated human fetal parathyroid tissue produced with purified and unpurified alginate were xenografted into the peritoneal of mice which were divided into 4 groups. Transplanted empty microcapsules were established as control. Human parathyroid was measured to identify the secretory function of microcapsulated human parathyroid and the morphological changes of microcapsulated tissue were observed.ResultsTwo months after transplantation, there are significant differences in secreting function of microencapsulated parathyroid tissue between purified and unpurified groups(P< 0.05 ),( 38.8 ± 13.7 ) pg/ml and ( 1.2 ± 0.7 ) pg/ml respectively. The retrieve rates of purified and unpurified alginate empty microcapsules are (72±18)% and (24±13)%,(65±21)% and 0 respectively after 1 and 2 months of transplantation.ConclusionMicrocapsules produced with purified alginate have good biocompatibility, the survival time of parathyroid tissue in the purified microcapsules was prolonged significantly.

关 键 词：海藻酸钠 纯度 微囊纤维化 甲状旁腺组织功能 甲状旁腺移植 

分 类 号：R653[医药卫生—外科学] R977[医药卫生—临床医学]