高效液相色谱法测定染发剂中的12种有机胺类染料  

Determination of 12 kinds of organic amine dyes in hair dyes by HPLC method

作　　者：陈希[1,2] 张国文[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]江西省食品药品检验所,江西南昌330029

出　　处：《南昌大学学报（理科版）》2014年第2期143-146,152,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金项目(31060210;21167023);食品科学与技术国家重点实验室基金项目(SKLF-ZZB-201305;SKLF-ZZA-201302;SKLF-KF-201203)

摘　　要：建立染发剂中对苯二胺等12种染料的高效液相色谱-二极管阵列检测器(HPLC-DAD)测定方法。采用RP-AMIDE C16柱(4.6mm×250mm,5μm),流动相为磷酸盐混合溶液-乙腈(9010),流速为1mL·min-1,检测器为DAD,检测波长为280nm。样品用10%亚硫酸氢钠水溶液-乙醇(11)混合溶液冰水浴超声提取,离心过滤后检测。12种染料在给定的浓度范围内峰面积与浓度有良好的线性关系,相关系数均不低于0.999 7,平均回收率为98.1%～108.4%,相对标准偏差(RSD)为0.3%～1.4%,检出浓度为19～22μg·g-1。该方法简便、准确、快速,可用于检测染发剂中12种染料。A high-performance liquid chromatography with diode array detection （HPLC-DAD） method was developed for the determination of p-phenylenediarnine and other 11 kinds of dyes in the commercially available hair dyes. The HPLC was performed on a RP-AMIDE C16 column （4.6 mm×250 mm,5 μm） ,with phosphate mixed solution-acetonitrile（90 ： 10） as the mobile phase and detected by DAD with the detection wavelength of 280 nm. The flow rate was 1 mL · min ^-1. All the samples were extracted using ultrasonic techniques in the ice-water bath with 10% sodium bisulfite-ethanol（1 ： 1） mixed solution. The extract was centrifuged and filtered. In the given concentration range, the peaking areas and concentration of the twelve kinds of dyes had a good linear relationship,and the correlation coefficient was not less than 0. 999 7. The average recoveries were 98.1% -108.4 % with relative standard deviations of 0.3 % - 1.4 %, and the detection limits were 19-22 μg · g ^-. The method is simple,accurate and fast,which could be used for the determination of 12 kinds of dyes in hair dyes.

关 键 词：高效液相色谱法 二极管阵列检测器 染料 染发剂 

分 类 号：O657.72[理学—分析化学]