铜绿假单胞菌LCT-PA220菌株全基因组序列测定与分析  

Whole genome sequencing and analysis of Pseudomonas aeruginosa strain LCT-PA220

作　　者：刘超[1] 苏龙翔[1] 方向群[1] 刘长庭[1] 

机构地区：[1]解放军总医院南楼呼吸科,北京100853

出　　处：《解放军医学院学报》2014年第7期738-740,758,共4页Academic Journal of Chinese PLA Medical School

基　　金：国家"973"重点基础研究发展规划项目(2014CB744400);全军医学科研"十二五"课题重点项目(BWS12J046);武器预研重点基金(9140A26040312JB10078)~~

摘　　要：目的全基因组测序和分析铜绿假单胞菌LCT-PA220菌株,研究空间因素对细菌全基因组的影响,为宇航员的保健奠定基础。方法通过革兰染色鉴定LCT-PA220属性,提取基因DNA、构建文库并上机测序;加工处理原始测序数据后进行基因组组装、注释和分析。结果铜绿假单胞菌LCT-PA220菌株革兰染色阴性,基因组为6.87M,包括5 829个编码序列,平均长度为962 bp,预测到为54个tRNA基因,COG数据库和KEGG数据库比对共注释到22组COG功能和32条KEGG信号通路。结论铜绿假单胞菌LCT-PA220菌株全基因组测序和基因组清楚,为后续对该菌株的研究打下了基础。Objective To study the influence of the spatial factor which is closely associated with spacemen＇s health, the whole genome sequence of Pseudomonas aeruginosa strain LCT-PA220. Methods The characteristic was identified by gram stain examination. Then genomic DNA was extracted from LCT-PA220 strain and the library was constructed and followed by sequencing. The raw date were filtered, assembled into genome, annotated and analyzed. Results The identify of LCT-PA220 strain was gram-negative bacteria whose genome was 6.87M containing 54 tRNA genes and 5 829 coding sequences with 962 bp average gene length. COG and KEGG annotation analysis revealed 22 COG functions and 32 KEGG pathways, respectively. Conclusion The whole genome of LCT-PA220 strain is sequenced and annotated well, which paves the way for further study of this strain.

关 键 词：铜绿假单胞菌 基因组 测序 编码序列 

分 类 号：R378[医药卫生—病原生物学]