高效液湘色谱法测定苯、苯酚、对苯醌、苯二酚和苯三酚  

Determination of benzene and its oxidation products:phenol,benzoquinone,bihydroxybenzene and benzenetriol by high performance liquid chromatography

作　　者：额日和木[1] 王旭[1] 张煜琳 萨嘎拉[1] 照日格图[1] 

机构地区：[1]内蒙古师范大学化学与环境科学学院,内蒙古自治区绿色催化重点实验室,呼和浩特010022

出　　处：《分析仪器》2014年第3期34-39,共6页Analytical Instrumentation

基　　金：国家自然科学基金(NO.20966008);内蒙古自治区重大基础研究开放课题(2010KF02;20130902);内蒙古自治区高等学校产学研结合专项(NJCXY08020)项目资助

摘　　要：本文采用高效液相色谱法，以乙腈-水（50：50，v/v）混合溶液为流动相，经 YMC -C18（250 mm ×46mm ，5μm）色谱柱分离，紫外检测波长为270nm时，测定了苯、苯酚、对苯醌、邻苯二酚、间苯二酚、对苯二酚和苯三酚。结果表明：苯、苯酚、对苯醌、邻苯二酚、间苯二酚、对苯二酚和苯三酚在各自的测定范围内均有良好的线性关系（相关系数为≥0.999），方法的检出限为0.002～0.008μg/mL。内标法（ISM ）回收率为80.40％～101.59％，相对标准偏差＜9.6％；外标法（ESM ）回收率为82.72％～99.48％，相对标准偏差＜10.8％。说明在苯、苯酚、对苯醌、邻苯二酚、间苯二酚、对苯二酚和苯三酚定量分析方法中内标法优于外标法，并且该方法操作简便、快速、稳定性好、样品和溶液用量少，分析条件温和。Based on the high performance liquid chromatography method ,benzene and its direct oxida-tion products ：phenol , benzoquinone , o-Bihydroxybenzene , m-Bihydroxybenzene , p-Bihydroxybenzene , benzenetriol were completely separated by the YMC-C18（250mm × 46mm ,5μm） chromatographic column with acetonitrile-water（50：50 ,v/v） as mobile phase .The UV detection wavelength was set at 270 nm .The result showed good linearity （R≥0 .99） ,the detection limit of the samples were between 0 .002-0 .008 μg/mL .The recoveries of internal standard method（ISM） ranged from 80 .40 to 101 .59% at the three spiked levels ,the relative standard deviation was below 9 .6% ;the recoveries of external standard method （ESM ） ranged from 82 .72 to 99 .48% at three spiked levels ,the relative standard deviation was below 10 .8% .It was indicated that the internal standard method was better than the external standard method for the quan-titative analysis of benzene and its products of oxidation .T his method is simple ,rapid ,sensitive and stabi-lized ,low consumption of sample and solution ,and analytical conditions are moderate .

关 键 词：苯 苯酚 内标法 外标法 高效液相色谱 

分 类 号：O657.72[理学—分析化学]