HPLC法测定维生素B_4片的有关物质  

Determination of related substances of Vitamin B_4 Tablets by HPLC

作　　者：顾秋琴[1] 

机构地区：[1]泰州市食品药品检验所,泰州225300

出　　处：《海峡药学》2014年第5期43-45,共3页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定维生素B4片的有关物质。方法采用Waters SunFireTMC18柱(250mm×4.6mm,5μm),流动相为甲醇-0.05mol·L-1磷酸二氢钾溶液-三乙胺(10∶90∶0.25),流速0.8mL·min-1,检测波长261nm。结果主峰与各降解产物峰的分离良好,方法的精密度良好。结论本方法简便、准确、灵敏度高,可用于维生素B4片的有关物质检查。OBJECTIVE To establish a HPLC method to determine the related substances in Vitamin B 4 Tab-lets.METHODS The HPLC column was Waters SunFireTM C 18 （250mm ×4.6mm,5μm）;the mobile phase was methanol-0.05mol· L-1 potassium dihydrogen phosphate solution-triethylamine （10∶90∶0.25 ）;the flow rate was 0.8 mL· min-1 ,the detection wavelength was 261 nm.RESULTS Vitamin B4 was separated well from the related substances;The reproducibility and precision of the method were good.CONCLUSION The method is simple ,ac-curate and sensitive.It can be used for the determination of the related substances of Vitamin B 4 Tablets.

关 键 词：维生素B4片 高效液相色谱法 有关物质 

分 类 号：R927.2[医药卫生—药学]