间歇浸没式生物反应器快繁草莓脱毒苗的参数  

Micro-propagation of Fragaria×ananassa Duch.optimization of process parameters in temporary immersion bioreactors system

作　　者：张乔丽[1] 邵微[1] 高利梅[1] 姚允聪[1] 姬谦龙[1,2] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农学院生物科学与工程学院,北京102206

出　　处：《北京农学院学报》2014年第2期20-24,共5页Journal of Beijing University of Agriculture

基　　金：北京市自然科学基金项目;北京市教育委员会科技计划重点项目(KZ201210020018)

摘　　要：以茎尖脱毒处理诱导的草莓‘红颜’第3代继代苗为试验材料,利用间歇浸没式生物反应器进行组培苗增殖技术体系的研究。经过40d的增殖培养后,结果表明:(1)利用TIBs系统进行草莓组培苗培养增殖率约是传统培养方式的3倍;(2)TIBs系统中接种密度在2株/0.25L培养基/瓶,有利于草莓组培苗的生长;(3)TDZ和NAA质量浓度均为0.5mg/L时有利于TIBs系统中草莓组培苗的增殖;(4)TIBs系统中蔗糖质量浓度为30g/L较适宜;(5)荧光灯与红蓝LED灯对TIBs系统中草莓增殖无差异。A series of process parameters was established for micro-propagation of Fragaria×ananassa Duch.‘Benihoppe’ in a temporary immersion bioreactor system （TIBs）. Results of a 40-day multiplication culture showed that： （1） the proliferation rate is about twice higher than that by traditional solid gel based methods; （2） a seeding density of 2 plantlets per 0.25 L medium in a 500 mL bottom showed better proliferation rate than other treatments; （3） a better proliferation rate in TIBs was observed in a medium with TDZ and NAA concentrations of 0.5 mg / L respectively; （4） sucrose concentration of 30g / L was more appropriate in TIBs system; （5） light source showed no significant difference between fluorescent lamp and LED light for strawberry proliferation in TIBs.

关 键 词：间歇浸没式生物反应器(TIBs) 草莓'红颜’ 组培快繁 

分 类 号：S668.4[农业科学—果树学]