DNMT1对RA模型大鼠FLS凋亡的影响  

Effects of DNMT1 on FLS Apoptosis in RA Model Rats

作　　者：缪成贵[1] 周国梁[1] 陈建中[1] 刘健[1] 

机构地区：[1]安徽科技学院,安徽蚌埠233000

出　　处：《畜牧与饲料科学》2014年第5期13-15,共3页Animal Husbandry and Feed Science

基　　金：安徽科技学院校级重点建设课程项目(ZD-KC1136);国家自然科学基金(81302783);安徽省高校自然科学研究项目(KJ2013B082);安徽科技学院研究项目(ZRC2013378);在滁高校专利技术研究项目(201306);安徽省教育厅项目(KJ2012Z069)

摘　　要：[目的]研究DNA甲基转移酶1(DNMT1)对类风湿性关节炎(RA)模型大鼠关节滑膜成纤维样滑膜细胞(FLS)凋亡的影响。[方法]采用Real-time qPCR检测对照大鼠和RA模型大鼠FLS中DNMT1表达,检测DNMT1 siRNA转染对抗凋亡基因Bcl-2表达的影响;采用MTT检测DNMT1 siRNA转染对FLS增殖的影响。[结果]与对照组相比,RA模型大鼠FLS中DNMT1表达显著升高,DNMT1表达抑制后,Bcl-2表达显著降低;MTT检测发现,DNMT1表达抑制后,FLS增殖活力显著减弱。[结论]DNMT1表达抑制可能引发RA模型大鼠FLS凋亡。[ Objective ] The effects of DNA methyltransferase 1 （DNMT1） on the apoptosis of fibroblast-like synovial cells （FLS） in rheumatoid arthritis（RA）model rats were studied. [Method] The expression of DNMT1 in RA model rats and control rats was detected by using Real-time qPCR. And the effects of siRNA transfection of DNMT1 on the expression of anti-apoptosis gene Bcl-2 were studied. The effects of siRNA transfeetion of DNMT1 on the proliferation of FLS were detected by MTY. [Result] Compared with control group, the expression of DNMT1 in FLS of RA model rats was significantly up-regulated. After the expression of DNMT1 was inhibited,the expression of Bcl-2 was significantly down-regulated. The results of MTF detection showed that the proliferation activity of FLS was significantly reduced after the inhibition of DNMT1 expression. [ Conclusion ] The inhibition of DNMT1 expression might lead to the apoptosis of FLS in RA model rats.

关 键 词：DNA甲基转移酶1 类风湿性关节炎 成纤维样滑膜细胞 Bcl-2 

分 类 号：R593.22[医药卫生—内科学]