育真熄风汤对PD模型小鼠多巴胺能神经元的作用  

Effect of Yzhenxifeng decoction on dopaminergic neurons in PD mice

作　　者：秦博文[1] 党晓伟[1] 卢思英 商亚珍[2] 吕瑞芳[1] 

机构地区：[1]承德护理职业学院,河北省067000 [2]承德医学院

出　　处：《江苏医药》2014年第11期1249-1251,F0002,共4页Jiangsu Medical Journal

基　　金：河北省教育厅高等学校科学技术研究指导项目(Z2012074)

摘　　要：目的探讨育真熄风汤对帕金森病(PD)模型小鼠多巴胺(DA)神经元的影响。方法用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导小鼠PD模型,随机分为空白对照(A)组、模型对照(B)组、中药治疗(C1-C3)组和阳性对照(D)组,采用爬杆测试法观察育真熄风汤对PD小鼠行为变化的影响,免疫组化染色法观察育真熄风汤对小鼠黑质内酪氨酸羟化酶(TH)阳性细胞表达的变化。结果与B组比较,C组运动协调障碍有所减轻,DA神经元数目增多,TH表达增强。结论育真熄风汤具有改善MPTP诱导的PD模型小鼠的运动障碍,保护DA神经元的作用。Objective To study the protective effects of Yuzhenxifeng decoction on the dopaminergic neurons in Parkinson＇s disease （PD） mouse model. Methods PD mice models were induced by 1-methyl-4-phenyl-5-four hydrogen pyridine （MPTP）, which were assigned into four groups of A （ blank control）, B （model control）, C （treated with Yuzhenxifeng decoction） and D （positive drug cobtrol）. The behavior changes were observed by climbing pole. The changes of expression of tyro,sine hydroxylase（TH） in substantia nigra were observed using immunohi,stochemical staining technique. Results Compared with group B, motor coordination disorder, dopamine neurons and expression of TH were enhanced in group C, Conclusion Yuzhenxifeng decoction can improve dyskine,sia of MPTP-induced PD mice and protect dopamine neurons.

关 键 词：育真熄风汤 帕金森病 多巴胺神经元 

分 类 号：R285[医药卫生—中药学]