小檗碱对3T3-L1脂肪细胞炎症因子、脂肪因子及脂肪酸代谢的影响  

Effects of Berberine on Inflammatory Factors,Adipokines and Fatty Acid Metabolism in 3T3-L1 Adipocytes

作　　者：李萍[1] 岳晶晶[2] 张达[3] 牛文彦[3] 何庆[1] 

机构地区：[1]天津医科大学总医院内分泌科,300052 [2]安徽省五河县人民医院内2科 [3]天津医科大学免疫系

出　　处：《天津医药》2014年第6期513-516,共4页Tianjin Medical Journal

基　　金：国家自然科学基金(项目编号:81170740);天津市科委科技支撑计划项目(项目编号:09ZCZDSF04500)

摘　　要：目的：观察小檗碱对3T3-L1脂肪细胞炎症因子、脂肪因子及脂肪酸代谢的影响，探讨小檗碱改善胰岛素抵抗的分子机制。方法3T3-L1脂肪细胞经不同浓度小檗碱（0、5、10、20和40μmol/L）处理24 h后，采用qRT-PCR技术检测相关因子mRNA表达水平，包括白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、瘦素、脂联素和内脂素以及脂肪酸合酶（FAS）、乙酰辅酶A羧化酶（ACC）、脂肪组织甘油三酯水解酶（ATGL）、脂肪细胞型脂肪酸结合蛋白（AFABP）。同时用10μmol/L小檗碱分别处理脂肪细胞0、4、8、24、48 h后，以相同方法测定上述指标mRNA表达水平。结果10-40μmol/L小檗碱剂量组的脂肪细胞内IL-6、TNF-α、瘦素、FAS、ATGL、AFABP mRNA表达水平均低于0μmol/L组，内脂素mRNA高于0μmol/L组（P〈0.05），各组间脂联素mRNA表达无明显差异（P〉0.05）。8-48 h时间处理组细胞内IL-6、TNF-α、瘦素、AFABP、ATGL、FAS mRNA表达水平均低于0 h组，内脂素mRNA水平高于0 h组（P〈0.05）。脂联素mRNA表达水平仅在48 h处理组降低（P〈0.05）。各组间ACC mRNA表达无明显差异（P〉0.05）。结论小檗碱能影响3T3-L1脂肪细胞炎症因子、脂肪因子和关键脂肪酶及蛋白mRNA的表达，且呈时间、剂量依赖效应，这可能是其改善胰岛素抵抗的重要分子机制。Objective To observe the effects of berberine on inflammatory factors, adipokines and fatty acid metabo-lism in 3T3-L1 adipocytes, and to investigate the molecular mechanism underlying berberine’s role of improving insulin re-sistance. Methods mRNA level of inflammatory molecules, adipokines, key enzymes and protein in fatty acid metabolism in 3T3-L1 cells were determined by quantitative real time polymerase chain reaction （qRT-PCR） after cells were treated with different concentrations of berberine （0, 5, 10, 20, 40μmol/L） for 24 hours and with 10μmol/L berberine at different du-rations （0,4,8,24,48 h）. These factors mainly included interleukin-6 （IL-6）, tumor necrosis factor-α（TNF-α）, leptin, adipo-nectin, visfatin, fatty acid synthase （FAS）, acetyl-CoA carboxylase （ACC）, adipose triglyceride lipase （ATGL） and adipocyte fatty acid binding protein （AFABP）. Results In 3T3-L1 adipocytes, transcription level of IL-6, TNF-α, leptin, FAS, AT-GL, AFABP reduced with addition of berberine dosage at 10-40μmol/L（P〈0.05）while visfatin mRNA level increased（P〈0.05）compared with the control group. No significant difference was found in expression of adiponectin（P〉0.05）. Tran-scription level of IL-6, TNF-α, leptin, AFABP, ATGL, FAS decreased with time after 10μmol/L berberine intervention （8-48 h） compared with the control group（P〈0.05）. On the other hand, visfatin mRNA level increased（P〈0.05）compared with the control group. Adiponectin mRNA decreased only after cells were treated with berberine for 48 h（P〈0.05）. No sig-nificant difference was found transcription of ACC between each groups treated with berberine（P 〉 0.05）. Conclusion mRNA level of inflammatory factors, adipokines, key enzymes and protein in fatty acid metabolism in 3T3-L1 adipocytes can be affected by berberine and this effect depend on its dose and time . This might be the mechanisms underlying berber- ine to improve insulin resistance.

关 键 词：小檗碱 胰岛素抵抗 白细胞介素-6 肿瘤坏死因子α 瘦素 脂联素 内脂素 乙酰CoA羧化酶 脂肪酸合酶 脂肪组织甘油三酯水解酶 脂肪细胞型脂肪酸结合蛋白 基因表达 INTERLEUKIN-6 

分 类 号：R587.1[医药卫生—内分泌]