脑室内注射瘦素对四肢骨折愈合的影响  

Effect of Intracerebroventricular Leptin Injection on Limbs Fracture Healing

作　　者：刘保龙[1] 孙志明[2] 武俏丽[2] 王宏[2] 闫华[2] 

机构地区：[1]天津医科大学研究生院,300070 [2]天津市环湖医院神经外科

出　　处：《天津医药》2014年第6期558-560,共3页Tianjin Medical Journal

基　　金：天津市科学技术委员会基金课题(项目编号:13JCYB-JC21100);天津市卫生局攻关课题(项目编号:10KG208)

摘　　要：目的观察兔胫腓骨骨缺损模型脑室内注射瘦素蛋白(leptin)对骨折愈合速度的影响。方法建立新西兰兔右侧胫腓骨外侧骨缺损模型,leptin组经兔枕骨大孔向小脑延髓池脑脊液中注射重组兔leptin,对照组以相同方法注射等剂量生理盐水。于术后1、3、7、14、21 d记录兔体质量变化,并通过CT动态记录骨缺损区域骨痂骨密度(BMD)的变化。于术后21 d,所有动物均处死后取右侧胫腓骨骨缺损区域,以荧光原位杂交技术(FISH)检测骨折区域骨形成蛋白(BMP)-2的表达情况。结果 2组动物造模后,体质量先下降后(14 d)上升,BMD一直呈上升趋势;术后1、3 d,2组体质量及BMD差异均无统计学意义,术后7、14、21 d,leptin组的体质量低于对照组,BMD高于对照组(均P<0.05)。术后21 d,骨缺损区域BMP-2表达阳性细胞数目高于对照组(个:120.87±29.14 vs 97.65±20.97,t=6.79,P<0.001)。结论经枕骨大孔向小脑延髓池内中注射leptin可加速兔四肢骨缺损的愈合。Objective To investigate the enhanced osteogenesis during bone fracture healing after intracerebroven-tricular （ICV） leptin injection, using rabbit model with created segmental bone defect in right tibial. Method Segmental critical-sized defects were created at the right tibial bone of skeletally mature New Zealand white rabbits. In experiment group （leptin group）, recombinant rabbit leptin was injected into cerebellomedullary cistern through foramen magnum. While in control group, normal saline was injected in the same way. Bone Mineral Density （BMD） was evaluated by qCT at 1st, 3rd, 7th, 14th and the 21st days. At the 21st days, all rabbits were euthanized to collect the right tibia for histomorphology, to ex-amine the BMP-2 expression in the bone callus by Fluorescence in situ hybridization （FISH）. Result In the leptin group, body weight declined more obviously than control group then it start to arise at the 14th day;qCT showed significant higher BMD in leptin group than in control group at the 7th day;FISH showed a higher BMP-2 expression in leptin group than in control group. Conclusion Cerebellomedullary leptin injection through foramen magnum could accelerate limb fracture healing in rabbit model with right tibial bone defection.

关 键 词：瘦素 脑脊液 胫骨骨折 骨折愈合 骨密度 原位杂交 荧光 兔 骨形成蛋白2 BONE morphogenetic protein 2 

分 类 号：R683.4[医药卫生—骨科学]