小鼠脊髓损伤后周细胞促进脊髓血管新生  

Pericyte mediated angiogenesis in spinal cord injury mice

作　　者：荆瀛黎[1] 武清斌[1] 苑晓晨[1] 李炳蔚[1] 刘明明[1] 张晓艳[1] 刘淑英[1] 李宏伟[1] 修瑞娟[1] 

机构地区：[1]中国医学科学院北京协和医学院卫生部微循环重点实验室微循环研究所,北京100005

出　　处：《基础医学与临床》2014年第6期828-833,共6页Basic and Clinical Medicine

基　　金：协和研究生创新基金(2012-1001-017)

摘　　要：目的探讨脊髓损伤后周细胞对微血管的影响。方法 C57BL/6小鼠随机分为:假手术组、损伤后2、7和14 d组(S2、S7和S14),每组9只。损伤组采用改良Allen's法制作中度脊髓损伤模型。给小鼠颈静脉注入番茄凝集素(LEA),检测灌注微血管面积;用免疫荧光和免疫组化检测微血管面积和数目;用免疫荧光检测周细胞覆盖率。用缺氧条件(95%N2,5%CO2)模拟脊髓损伤的病理条件,用基质胶系统检测内皮细胞成管。结果与假手术组相比,S2组灌注血管面积、微血管面积和数目显著降低(P<0.001)。与S2组相比,S7组微血管数目和面积显著增高(P<0.05);S14组灌注血管面积、微血管数目和面积均显著增高(P<0.05,P<0.01),但平均值仍低于假手术组。与假手术组相比,S2和S7组周细胞覆盖率持续下降(P<0.001)。周细胞与内皮细胞共培养可显著增加缺氧条件下内皮细胞成管长度(P<0.01)。结论脊髓损伤后周细胞可促进脊髓血管新生。Objective To investigate the influence of pericytes on microvascular angiogenesis after spinal cord injury （SCI）. Methods C57BL/6 mice were randomly divided into four groups： sham group, 2 days （S2）, 7 days （$7） and 14 days （S14） after SCI group （n = 9 per group）. The injury group received a moderate impacted spinal cord injury. Perfused blood vessels were detected by FITC-LEA intravenous injection; mierovessel area density （MVA） and microvessel density （MVD） were analyzed by immunofluoreseence and immunohistochemistry, respectively; pericyte coverage was further calculated by immunofluoreseence. Anoxie condition （95% N2 and 5% CO2 ） was applied to mimic the pathological profile of SCI, endothelial tubular formation was detected by matrigel system. Results The area of perfused blood vessels, MVA and MVD at S2 were significantly decreased compared with that at sham （P 〈 0. 001 ）. MVA and MVD at S7 was notably increased compared with that at S2 （P 〈 0.05）. The area of peffused blood vessels, MVA and MVD at S14 showed a markedly increase compared with that at S2 （P 〈 0. 05, P 〈0.01 ） , which were still lower than that at sham. Pericyte coverage was decreased at S2 and S7 in mice compared with sham control. Co-culture of pericytes and endothelial cells（ECs） could markedly increase endothelia tube length compared with ECs alone under anoxia（ P 〈 0.01 ）. Conclusions Pericytes might promote angiogenesis in spinal cord injury C57BL/6 mice.

关 键 词：周细胞 脊髓损伤 血管新生 

分 类 号：R651.2[医药卫生—外科学]