当年生喜树种子的无菌发芽及幼苗生长  

Aseptic germination and seedling growth of Camptotheca acuminate seeds on that very year

作　　者：徐颖莹 黄明亮[1] 单梅[1] 胡家金[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2014年第3期277-280,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省教育厅项目(08C430)

摘　　要：以当年生新鲜喜树种子为材料，采用植物组织培养技术，研究其萌发及幼苗生长的适宜条件。结果表明，采用0．1％HgCl2和10％NaClO分别对外植体进行消毒后，以0．1％HgCl2进行果实消毒的种子萌发率最高，为41．11％；在此基础上用10％NaClO将预培养种子浸泡3min后，其萌发率和成苗率显著提高，分别达85．56％和55．56％，而且幼苗生长状态好，平均生成侧根6～7条，主根长约10cm；对未经处理的种子苗做切根处理，并转移到新鲜的MS培养基，幼苗生长效果与经10％NaClO处理过的种子苗的生长效果相同，但将其转移到含1mg／L 6-BA的MS培养基后，种子苗的子叶变黄，无新根生成或只有1个短根生成。The suitable condition of seeds germination and their growth were researched by taken fresh seeds of Camptotheca acuminate on that very year as materials through plant tissue culture. To compare the rate of seed germination, explants were disinfected with 0.1% HgCl2 and 10% NaClO respectively. The result showed that rate of seed germination was the highest with 0.1% HgCl2 disinfection, which achieved 41.11%. Further, rate of seed germination and seedling were significantly increased with 10% NaClO soak for 3 minutes on the basis of treatment of 0.1% HgCl2 disinfection, which reached 85.56% and 55.56% respectively. Fresh seedlings grew well with an average of 6-7 lateral roots and a 10 cm long taproot. The growth of seedlings treated by root-cut and transferred in fresh MS medium were same as treatment with 10% NaClO. Seedlings, however, became yellow and had no new root or only one short root after transferred them to MS medium with 1 mg/L 6-BA.

关 键 词：喜树 种子 萌发率 成苗率 切根 

分 类 号：Q945.34[生物学—植物学]