一株产赖氨酸芽孢杆菌的筛选  

Screening of Bacillus spp. Producing Lysine

作　　者：邢月腾[1,2] 李丽立[2] 王升平[2] 范觉鑫[1,2] 林彬彬[1,2] 欧荣娣[1,2] 张彬[1] 

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]中国科学院亚热带农业生态研究所动物生态营养与健康养殖联合实验室,湖南长沙410125

出　　处：《养猪》2014年第3期9-10,共2页Swine Production

基　　金：国家自然科学基金项目(31272462);湖南省科技计划重点项目(2100CK2006);长沙市科技计划重点项目(K1201108-21)

摘　　要：87株芽孢杆菌经过2次菌液上清液赖氨酸含量测定,筛选出1株赖氨酸产量较高的菌株YB83,其在LB上清发酵液中赖氨酸含量达186.85μg/mL,比对照组提高27.68%,该菌通过菌落形态、生理生化特征和分子生物学鉴定为枯草芽孢杆菌。87 strains of Bacillus spp. as initial bacteria were applied to select and breed high-yield lysine strain. After twice screening by determining the lysine content of bacterial supermatant,one strain named YB83 was screened out,whose lysine content was 186.85 μg/mL,27.68% higher than LB control medium.According to the morphological and physiochemical properties,combining with 16S rDNA gene sequence and its position in the phylogenetic tree,strain YB83 was finally identified as Bacillus subtilis.

关 键 词：芽孢杆菌 赖氨酸 筛选 

分 类 号：S816.7[农业科学—饲料科学]