油桐花芽2个不同发育时期转录组分析  

The Sequencing Analysis of Transcriptome of Vernicia fordii Flower Buds at Two Development Stages

作　　者：孙颖[1] 谭晓风[1] 罗敏[1] 李建安[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室经济林培育与保护省部共建教育部重点实验室,长沙410004

出　　处：《林业科学》2014年第5期70-74,共5页Scientia Silvae Sinicae

基　　金：国家林业公益性行业科研专项重大项目(201204403)

摘　　要：利用RNA-seq技术对油桐2个不同发育时期的花芽的转录组进行比较分析,获得大量差异表达的unigene序列。通过GO分类和Pathway富集性分析,将这些差异表达unigene分别归类于55个GO term和128个代谢途径。unigene中有103个与植物开花相关,涉及4个开花调控途径(光周期途径、春化途径、GA途径和自主途径)。随着花芽发育,越来越多的unigene表达量呈现上调趋势,上调的基因数量超过下调基因数量。研究结果可在一定程度上解析油桐花芽形态分化的分子调控模式与机制。This research compared transcriptome of Vernicia fordii flower buds at two different developing stages using RNA-seq technique and then obtained a lot of differentially expressed unigenes.Through GO classification and pathway enrichment analysis,all of these differentially expressed unigenes were classified into 55 GO terms and 128 metabolism pathways,respectively.One hundred and three unigenes identified from transcriptome data were referred to four major plant flowering regulation pathways including photoperiod pathway,verbalization pathway,GA-dependent pathway and autonomous pathway.With the development of flower buds,more and more unigenes＇ expression levels were up-regulated and the number of up-regulated genes was more than that of down-regulated genes.This research has resolved to some extent the molecular mechanisms regulating mode with flower bud morphological differentiation of V.fordii.

关 键 词：油桐 花芽 转录组测序 开花基因 

分 类 号：S718.46[农业科学—林学]