油松成熟胚培养不定芽状态对其生根状况的影响  

Effect of the Status of Adventitious Buds In Vitro Cultured from Mature Embryo of Pinus tabulaeformis on Its Rooting

作　　者：买凯乐[1] 邵砾群[2] 李荣珍[1] 董丽芬[2] 兰健花[1] 

机构地区：[1]广西生态工程职业技术学院,柳州545004 [2]西北农林科技大学,杨凌712100

出　　处：《林业科学》2014年第5期147-153,共7页Scientia Silvae Sinicae

基　　金：国家林业局项目"干旱地区主要造林树种快速育苗及设备研究"(96-122);广西生态工程职业技术学院学院立项课题(201105A);广西高校科学技术研究项目(2013LX199)

摘　　要：油松（Pinus tabulaeformis）是我国特有的针叶树种，木材坚实，富含松脂，耐腐朽，是优良的建筑、电杆、枕木、矿柱等用材。油松是北方的主要造林树种之一，它分布广，适应性强，根系发达，树姿雄伟，枝叶繁茂，有良好的保持水土和美化环境的功能（徐化成，1993）。The mature embryo of Pinus tabulaeformis was used as explants to induce adventitious buds and root on modified 1/2MS medium. The results showed that the largest multiplication rate with strong buds was obtained when adventitious bud cluster was divided into three pieces. The rooting rate of adventitious buds was 91.7%. The adventitious bud clusters with diameter larger than 0.9 cm, and the shoots with length longer than 6 mm had higher rooting rate. In the process of shoot elongation and muhiplication, short culture duration could lead to a higher rooting capacity of the adventitious bud cluster, and the 28-days culture period was optimal for rooting, leading to a rooting rate of 73.3%. Adventitious bud clusters with big biomass were easily to root, and the higher the rooting rate the bigger the bud cluster biomass.

关 键 词：油松 成熟胚 改良1 2MS培养基 不定芽诱导 不定芽生根 

分 类 号：S723.13[农业科学—林木遗传育种]