小鼠急性酒精性肝损伤模型的制备及观察  

The Establishment and Observation of Alcohol-induced Acute Liver Injury Model in Mice

作　　者：林晓晖[1] 黄玲[1] 王一铮[1] 刘如玉[1] 

机构地区：[1]福建中医药大学中西医结合学院,福州350122

出　　处：《北方药学》2014年第6期67-69,共3页Journal of North Pharmacy

摘　　要：目的:建立小鼠酒精性肝损伤模型,并动态研究小鼠血清和肝脏的生化指标的变化。方法:将80只清洁级ICR小鼠随机分为8个组:4个模型组和4个空白组。模型组小鼠按12ml·kg-1d-1胃灌注52度白酒,空白组小鼠给予胃灌注等量蒸馏水,分别在灌胃后2d,4d,6d,8d各取一组模型组和空白组小鼠,测定血清ALT、TG,肝脏指数,肝组织SOD和MDA。结果:与同时段空白组比较,造模6d后,小鼠血清中ALT和TG均显著升高(P<0.01),肝脏指数、肝组织中TG和MDA显著升高(P<0.01),而SOD活性显著下降(P<0.01)。结论:本实验在短时间内成功建立小鼠急性酒精性肝损伤模型,稳定性好,可应用于护肝药物保护机制的研究。Objective：To establish an alcohol-induced acute liver injury model in mice and to dynamically monitor the changes of biochemical indexes in serum and liver. Methods ：Eighty ICR mice were randomly divided into eight groups, four control groups and four model groups. The model groups were given 52%（V/V）alcohol with 12ml·kg^-1d^-1 by gastric perfusion while the control groups were given distilled water. At 2, 4, 6, 8 days after administration, a model group and a control group were respectively sacrificed to take serum samples for measurements of ALT and TG levels, and liver samples for measurements of liver index, TG, SOD and MDA levels. Results：After treating for 6 days, serum ALT and TG levels were significantly increased（P〈0.01）, and liver index, TG and MDA level in liver tissue were increased significantly（P〈0.01）, whereas SOD level in liver tissue decreased significantly（P〈0.01）. Conclusion ：A mice model of alcohol-induced acute liver injury has been successfully established, which is stable and can be used for studying the mechanism of drug protection in alcoholic liver disease.

关 键 词：酒精性肝损伤 动物模型 

分 类 号：R595.6[医药卫生—内科学]