男性特发性无精症病人YRRM1基因的检测  

Detection of YRRM1 gene in azoospermia

作　　者：李怡[1] 郝冰涛[1] 杨艳丽[1] 王应太[1] 赵晶石[2] 廖世秀[1] 司艳梅[1] 

机构地区：[1]河南省人民医院遗传研究所,河南郑州450003 [2]河南省人民医院检测中心,河南郑州450003

出　　处：《河南医学研究》2001年第1期22-23,共2页Henan Medical Research

摘　　要：目的 :建立对男性无精症病人YRRM1的基因缺失诊断方法。方法 :运用多聚酶链式反应对 10例男性无精症或严重少精症的患者进行YRRM1基因位点缺失检测。结果 :10例发现 1例YRRM1基因发生缺失 ,其它病例未发现。结论 :YRRM1基因位点检测特异性位点的缺失和男性无精症有一定的相关性 。Objective: In order to establish a method to detect the abnormal YRRM1 gene in azoospermia.Methods: We Collected ten azoospermia persons and got the DNA with phenol chloroform.Using the special primer for YRRM1,the YRRM1 gene was analyzed by polymerase chain reaction and agar gel electrophoresis method.Results: No amplification band of YRRM1 gene was noted in 1 of 10 azoospermia person(10%).Conclusion: The deletion of YRRM1 gene may result in male infertility.We can use the method to detect partial pathogeny in azoospermia.

关 键 词：生精因子 YRRM1 多聚酶链式反应 男性不育症 

分 类 号：R698.2[医药卫生—泌尿科学]