酵母双杂交筛选与杨树Subgroup 4 MYB转录因子相互作用的蛋白质  

Identification of Subgroup 4 MYB transcription factorinteractive proteins using yeast two-hybrid system

作　　者：宫宇[1,2] 魏建华[2] 张少斌[1] 王宏芝[2] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]北京市农林科学院/北京农业生物技术研究中心,北京100097

出　　处：《广东农业科学》2014年第11期157-162,共6页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31270593);国家"973"计划项目(2012CB114501)

摘　　要：MYB转录因子在次生细胞壁合成转录调控过程中发挥重要的作用。为了深入研究杨树Subgroup 4MYB转录因子的作用机制,应用酵母双杂交的方法,以杨树Subgroup 4 MYB基因Poptr_0004s18020.1(MYB221)、Poptr_0009s13640.1(MYB156)和Poptr_0019s00750.1(MYB057)为诱饵蛋白,从毛白杨次生维管组织均一cDNA文库中筛选互作蛋白,获得了9个与Subgroup 4 MYB互作的蛋白质,并通过生物信息学方法分析候选蛋白可能的生物学功能。为进一步研究杨树Subgroup 4 MYB转录因子的作用机制奠定了基础。MYB transcription factors play important roles in transcriptionally regulating the synthesis of secondary cell wall. To further investigate the roles of Subgroup 4 MYB transcription factors in poplar, yeast two-hybrid system was used to find out the interactive proteins. The recombinants pDHB1-MYB057, pDHB1-MYB156 or pDHB1-MYB221 plasmids were used as bait plasmid to screen a cDNA library constructed from Populous secondary vascular tissue, and 9 interactive proteins were gotten. The potential functions of the candidate interaction proteins were analyzed using bioinformatics methods. This work will help to clarify the role of subgroup 4 MYB transcription factors in the synthesis of secondary cell wall in plants.

关 键 词：MYB转录因子 酵母双杂交 互作蛋白 

分 类 号：Q946.1[生物学—植物学]