线粒体部分敲除对氡染毒人支气管上皮细胞损伤效应的影响  

Effects of Mitochondrial DNA Partial Knockout on Injury of Human Bronchial Epithelial Cells Induced by Radon Exposure

作　　者：陈玉之 李冰燕[1] 孙静[1] 陈丽莉[1] 韦晔[1] 童建[1] 

机构地区：[1]苏州大学公共卫生学院,江苏215123

出　　处：《环境与职业医学》2014年第6期438-441,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金国际重大合作项目(编号:81020108028)

摘　　要：[目的]观察线粒体部分敲除对氡染毒人支气管上皮细胞损伤效应的影响。[方法]分别对线粒体DNA部分敲除的人支气管上皮细胞(ρ-HBE细胞)和未经敲除的ρ+HBE细胞暴露20 000 Bq/m3的氡及氡子体,观察两种受试细胞第10代(低剂量组,ρ+HBE-Rn10和ρ-HBE-Rn10)及第30代(高剂量组,ρ+HBE-Rn30和ρ-HBE-Rn30)细胞的增殖能力、细胞周期以及活性氧(ROS)水平的变化。[结果]氡染毒后,线粒体部分敲除后高、低剂量组(ρ-HBE-Rn30,ρ-HBE-Rn10)的细胞克隆形成率(PE)和细胞相对存活分数(SF)均高于敲除前(P<0.05);敲除前高剂量组(ρ+HBE-Rn30)的PE和SF均低于低剂量组(ρ+HBE-Rn10)(P<0.05),敲除后则高于低剂量组(P<0.05)。低剂量组ρ+HBE细胞中的S期细胞减少,G2/M期细胞增加,高剂量组的S期细胞增加,G1期细胞减少;各染毒剂量的ρ+HBE细胞和ρ-HBE细胞,ROS水平均升高(P<0.05)。[结论]经氡染毒后,ρ-HBE细胞在生长速度、细胞周期、ROS产生量等生物学指标上与ρ+HBE细胞有明显改变,表明氡所产生的细胞损伤效应与线粒体的结构和功能障碍密切相关。[ Objective ] To observe the impact of mitochondrial DNA partial knockout on the injury of human bronchial epithelial cells exposed to radon gas. [ Methods 3 Bronchial epithelial ceils with （p-HBE ceils） or without （p＋HBE ceils） mitochondrial DNA partial knockout were exposed to 20 000 Bq/m3 radon and radon daughters to observe the changes in cell proliferation, cell cycle, and intracellular reactive oxygen species （ROS） levels of cells at 10 passages （low-dose groups： p＋HBE-Rn10 and p-HBE-Rn10） and 30 passages （high-dose groups： p＋HBE-Rn30 and p-HBE-Rn30）. [ Results ] After radon exposure, the plating efficiency （PE） and the survival fraction （SF） of the p-HBE-Rnl0 and p-HBE-Rn30 cells were higher than those of the p＋HBE ceils （P 〈 0.05）. PE and SF were lower in the p＋HBE-Rn30 cells than the p＋HBE-RnlO cells （P 〈 0.05）, while higher in the p-HBE-Rn30 ceils than the p-HBE-Rn10 ceils （P〈0.05）. S phase decreased and G2/M phase of the p＋HBE-Rnl0 ceils increased, while the p＋HBE-Rn30 cells showed the opposite effects. The ROS levels were increased in all exposure groups. [ Conclusion ] Compared to the p＋HBE cells, the p-HBE cells have a significant change in cell proliferation, cell cycle, and intraceilular ROS generation after radon exposure, indicating that the radon induced cell injury is associated with mitochondrial structure and impairment.

关 键 词：氡 线粒体 肺癌 细胞增殖 细胞周期 

分 类 号：R114[医药卫生—卫生毒理学]