孕前慢性应激后仔鼠学习记忆与胰岛素样生长因子Ⅱ表达相关性研究  

Association of Learning and Memory with Expression of Insulin-Like Growth Factor-Ⅱ in Offspring Rats Following Progestational Maternal Chronic Stress

作　　者：关素珍[1] 夏迪亚.夏尔甫丁 徐仙[1] 刘继文[1] 

机构地区：[1]新疆医科大学公共卫生学院社会医学教研室,新疆830054

出　　处：《环境与职业医学》2014年第6期472-475,共4页Journal of Environmental and Occupational Medicine

基　　金：新疆维吾尔自治区青年科学基金项目(编号:2013211B50);新疆医科大学科研创新项目(编号:XJC201207)

摘　　要：[目的]观察孕前慢性应激后仔鼠学习记忆能力与海马中胰岛素样生长因子Ⅱ(insulin-like growth factor-Ⅱ,IGF-Ⅱ)的表达变化,探讨仔鼠学习记忆能力变化的机制。[方法]建立慢性应激孕前大鼠模型,通过血浆皮质酮动态测定来检测模型,采用Morris水迷宫和Y迷宫进行学习记忆能力测定、实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,RT-PCR)检测海马中IGF-Ⅱ表达水平。[结果]模型组母鼠血浆皮质酮水平增高(P<0.05);模型组所产仔鼠只数及母鼠孕天数均少于对照组(P<0.05),两组雌雄比例差异无统计学意义(P>0.05);模型组仔鼠血浆皮质酮高于对照组仔鼠(P<0.05);模型组仔鼠学习、记忆能力低于对照组(P<0.05);模型组仔鼠IGF-ⅡmRNA表达量低于对照组仔鼠(P<0.05)。[结论]孕前慢性应激后仔鼠空间学习记忆能力下降,可能与其脑内IGF-Ⅱ表达的下调及体内皮质酮升高有关系。[ Objective ] To observe=the learning and memory abilities and the insulin-like growth factor- Ⅱ （IGF-Ⅱ） expressions in hippocampus of offspring rats after maternal rats exposed to stress before pregnancy, and to explore the mechanism of learning and memory abilities＇ alteration in offspring rats. [ Methods ] A maternal rat model was established by using chronic mild stress stimulation protocol. Plasma cortisol levels of the maternal model rats were measured by radioimmuneassay. The abilities of learning and memory were determined using Morris water maze and Y maze, and the expressions of IGF- Ⅱ by real-time polymerase chain reaction. [ Results ] Compared with the control maternal rots, the plasma cortisol levels of the maternal model rats were increased （P〈0.05）. Compared with the controls, the number of the model offspring rats and the days of maternal pregnancy in pregnant rats were less （P〈 0.05）, but the male-to-female ratio was similar between the two groups （P〉 0.05）. The plasma cortisol levels of the model offspring rats were increased （P〈O.05）, while the learning and memory abilities （P〈0.05） and the GF-Ⅱ mRNA expression levels （P〈0.05） of the model offspring rats were decreased. [ Conclusion ] The progestational maternal stress exposure may reduce the learning and memory abilities in the offspring rats, which may be associated with the decreased expression of IGF- Ⅱ and the increased plasma cortisol level.

关 键 词：孕前 应激 仔鼠 学习记忆 皮质酮 胰岛素样生长因子Ⅱ 

分 类 号：R114[医药卫生—卫生毒理学] B842.3[医药卫生—公共卫生与预防医学]