内皮素-1对离体动脉的收缩作用及舒张药物的对抗效应  

Contractile effect of endothelin-1 on isolated artery and the antagonistic effect of vasodilators

作　　者：王涛[1] 顾玉东[1] 沈忆文[2] 

机构地区：[1]上海复旦大学附属华山医院手外科,200040 [2]上海复旦大学法医教研室

出　　处：《中华手外科杂志》2001年第1期34-35,共2页Chinese Journal of Hand Surgery

基　　金：美国中华医学会基金资助项目! (CMB 95 61 9)

摘　　要：目的　研究内皮素 1(Endothelin 1,ET 1)收缩血管的特性及显微血管外科常用舒张药物的对抗效应。方法　采用离体血管舒缩功能的实验技术比较ET 1与去甲肾上腺素 (noradrenaline ,NE)对离体动脉张力的影响 ,同时检测舒张药物对ET 1收缩血管的拮抗作用。结果　ET 1使离体大鼠颈总动脉环产生浓度依赖性的恒定收缩 ,ET 1与NE的半数有效浓度 (medianeffectiveconcentration ,EC50 )分别为 1.8× 10 -9mol/L和 3 .4× 10 -8mol/L ,NE的EC50 值为ET 1的 18.9倍。血管舒张药如利多卡因、硝苯啶、罂粟硷、硝普钠、山莨菪硷 ,能有效对抗ET 1的缩血管作用。其中以硝普钠最强 ,利多卡因与山莨菪硷较弱。结论　ET 1对离体动脉具有强大而持久的收缩效应。血管舒张药可以减弱ET 1的缩血管作用。Objective To observe the characteristics of vasoconstriction caused by endothelin - 1 and determine the antagonistic effect of vasodilators. Methods Vasomotory test of the isolated rat common carotid artery was done to compare the influence of endothelin - 1 (ET - 1) and noradrenaline (NE)on artery tension in vitro, and detect the antagonistic effects of vasodilators on ET - 1 induced vasoconstriction. Results Endothelin - 1 resulted in a constant constriction which was concentration - dependent in isolated rat common carotid arteries. The median effective concentration (EC 50 ) of endothelin - 1 and noradrenaline were 1.8 × 10 -9 mol/L and 3.4 × 10 -8 mol/L respectively. The EC 50 of noradrenaline was 18.9 times of that of endothelin - 1. Lidocaine, nifedipine, papaverine, sodium nitroprusside, and anisodamine all relaxed endothelin - 1 - induced vasoconstriction. The strongest among those vasodilators was sodium nitroprusside, whereas lidocaine and anisodamine weaker. Conclusions Endothelin - 1 induces potent and persistent vasoconstriction of isolated arteries. Vasodilators can reduce endothelin - 1 - induced vasoconstriction.

关 键 词：内皮缩血管肽-1 血管收缩 血管舒张药 药物拮抗作用 

分 类 号：R965[医药卫生—药理学]