建立乙烷硒啉在癌症患者血浆中的浓度测定方法及其药代动力学研究  

Determination of ethaselen concentration in cancer patients plasma and study on its pharmacokinetics

作　　者：陆开智 袁玮[2,3] 甘慧[2] 孟志云[2] 窦桂芳[1,2] 

机构地区：[1]广西医科大学研究生学院,南宁530021 [2]军事医学科学院野战输血研究所,北京100850 [3]沈阳药科大学生命科学与生物制药学院,沈阳110016

出　　处：《中国临床药理学杂志》2014年第6期521-523,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家十二五"重大新药创制"科技重大专项基金资助项目(2012ZX09301003-001-007)

摘　　要：目的建立湿法消解-电感耦合等离子体质谱法(ICP-MS)测定人血浆中乙烷硒啉的浓度,并将其用于乙烷硒啉分散片的人体药代动力学研究。方法血浆样品经硝酸消解后,用ICP-MS法测定给药后血浆中不同时间含硒药物总量,并用WinNonlin 5.2软件计算主要药代动力学参数。结果乙烷硒啉主要药代动力学参数如下:tmax为(9.20±0.98)h,Cmax为(597.58±221.73)ng·mL-1,AUC0-t为(2.57±0.92)μg·mL-1·h,平均滞留时间(MRT)为(24.60±0.63)h。结论建立的ICP-MS法简便、快速、灵敏,适用乙烷硒啉分散片临床监测。Objective To establish inductively coupled plasma mass spectrometry （ICP -MS ） method for determining the concentration of ethaselen in human plasma , and to apply it to the pharmacokinetic study of ethaselen dispersible tablets. Methods Plasma samples were digested with nitric acid, then detected by ICP- MS method. The main pharmacokinetic parameters were calculated with non -compartmental analysis by WinNonlin 5. 2 software. Results The parameters pharmacokinetic results of ethaselen were as follows： tmax was （9.20 ±0.98） h, Cmax was （597. 58±221.73） ng . mL^-1, AUC0-t was （2. 57±0. 92） ng . h . mL^-1, the mean residence time （MRT） was （24. 60 ± 0. 63 ） h. Conclusion The ICP- MS method is simple, rapid and sensitive, which is suitable for clinical determination of the concentration of ethaselen dispersible tablets in hu- inan plasma.

关 键 词：乙烷硒啉 电感耦合等离子体质谱法 血药浓度 药代动力学 

分 类 号：R969.1[医药卫生—药理学] R979.1[医药卫生—药学]