栀子苷对胰岛素抵抗HepG-2细胞葡糖转运体4表达的影响  

Dynamic effects of Geniposide on expression of glucose transporter 4 in insulin-resistance HepG- 2 cells

作　　者：戚琛晔[1] 席守民[1] 马灵筠[1] 周洁[1] 袁帅[1] 曹鹏涛[1] 李世朋[2] 

机构地区：[1]河南科技大学医学院,河南洛阳471003 [2]天津医科大学第一中心临床学院,天津300192

出　　处：《中国临床药理学杂志》2014年第6期537-539,共3页The Chinese Journal of Clinical Pharmacology

基　　金：2012年河南科技大学研究生创新基金资助项目(CXJJ-Z022)

摘　　要：目的研究栀子苷对胰岛素抵抗HepG-2细胞中葡萄糖转运蛋白4(GLUT4)表达的影响。方法用100 nmol·L-1胰岛素体外诱导培养法,建立HepG-2胰岛素抵抗细胞模型,随机分为对照组、栀子苷组和空白组,用RTPCR和细胞免疫化学技术,观察各组细胞在分别培养4,12,24,36 h后GLUT4的表达变化。结果经栀子苷处理过的HepG-2细胞中,GLUT4的表达量随时间的延长先增加后递减,在8 h时达到高峰。HepG-2细胞中GLUT4表达,栀子苷组与对照组比较差异有统计学意义(P<0.05)。结论栀子苷对HepG-2细胞胰岛素抵抗具有明显的改善作用,其作用机制可能与动态调节GLUT4的表达有关。Objective To investigate the effect of Geniposide on GLUT4 expression in insulin - resistance HepG - 2 cells. Methods After establish HepG- 2 cell model of insulin resistance by high concentrations of insulin - induced in vitro. The cell in model were randomly divided into three groups： control, Geniposide and blank groups. The expression changes of GLUT4 in insulin resistance HepG - 2 cells was observe at the time of 4, 12, 24, 36 h after culture by RT - PCR and immunocytochemistry. Results GLUT4 expression was first increased and then decreased with time in HepG- 2 cells after treated by Geniposide, and peaked at 8 h. However, compared with the control group, GLUT4 expression was significantly improved in Geniposide group. The difference was statistically significant （ P 〈 0. 05 ）. Conclusion The improving effect of Geniposide on the improvement of insulin - resistance of HepG - 2 cell in vitro is related to the expression of GLUT4.

关 键 词：栀子苷 HEPG-2细胞 胰岛素抵抗 葡萄糖转运蛋白4 

分 类 号：R285.5[医药卫生—中药学] R587[医药卫生—中医学]