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作 者:魏姗姗[1] 刘兴菊[1] 杨敏生[1] 梁海永[1]
机构地区:[1]河北农业大学,河北省林木种质资源与森林保护重点实验室,河北保定071000
出 处:《北方园艺》2014年第12期88-93,共6页Northern Horticulture
基 金:国家林业局林业公益性行业科研专项资助项目(201104039)
摘 要:以95份桃育成品种为试材,利用32对SSR分子标记对桃育成品种进行遗传多样性分析。结果表明:筛选的18对SSR引物共检测出93个等位基因,其中多态性等位基因为50个。桃群体的平均Nei’s基因多样度为0.5652,Shannon遗传表型指数为1.1125,说明桃总群体遗传变异偏低。针对桃的成熟期进行遗传分析,发现早熟和中熟类群遗传数据相似,推测二者亲缘关系相近;同理,推测晚熟品种与前二者关系较远。表明桃的成熟期与其等位基因相关,该研究结果可为进一步研究桃的遗传分析奠定基础。Taking 95 peach cultivars as materials, the genetic diversity of peach varieties using 32 pairs of SSR molecular markers were analyzed. The resuhs showed that 93 bands were amplified by 18 informative and reliable primers,of which 50 bands were polymorphic loci. A relatively low level of genetic diversity (Nei's gene diversity 0. 5652 and Shannon index 1.1125) was revealed at population level. For peach maturity,the experimental results for genetic analysis bad been taken,and the precocious and medium groups had similar genetic data had been found. In the same way, that the late maturity varielies had more distain relationship with both could be speculaten. The nmture differentiation of peach associated with allele frequency could be taken,this would help for the future indepth study genetic analysis of peach.
关 键 词:桃 简单重复序列(SSR) 遗传多样性 成熟期
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