香蕉假茎外植体愈伤组织诱导的初步研究  

Study of Banana Cauloid Explant Callus Induction

作　　者：欧克纬[1] 禤维言[1] 王兴伟[1] 冯斗[1] 

机构地区：[1]广西大学农学院,广西南宁530004

出　　处：《安徽农业科学》2014年第19期6167-6169,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究香蕉假茎外植体愈伤组织的最佳培养基。[方法]以香蕉组培无菌苗假茎为外植体,接种在含不同浓度配比激素的固体培养基中进行诱导培养,再将培养获得的胚性愈伤组织接种到液体培养基中进行悬浮培养。[结果]使用低浓度噻苯隆(TDZ)诱导出胚性愈伤组织的效果最好,最佳培养基为T2:MS+0.02 mg/L TDZ+7 mg/L 2,4-D。[结论]悬浮培养使用ZT2能较早建立ECS,且继代效果较好。[Objective] To study the optimum culture medium for banana cauloid explant callus induction.[Method] Using the banana cauloid which are germfree as the explants,inoculate them into the soil medium containing different kinds of the hormone.When we get the embryogenic callus,inoculate them into the liquid nutrient medium and conduct suspension culture.[Result] The test results showed that,when we use the TDZ with low concentration,the induction effects is the best.The best medium is T2：MS ＋ 0.02 mg/L TDZ ＋ 2,4-D 7 mg/L.[Conclusion] The best liquid nutrient medium is ZT2,as ZT2 could establish the ECS as soon as possible,and could get the best result.

关 键 词：香蕉(Musa nana Lour) 胚性愈伤组织 悬浮培养 

分 类 号：S188[农业科学—农业基础科学]