响应面法优化蔗渣酶解工艺研究  

OPTIMIZATION OF SUGARCANE BAGASSE HYDROLYSIS PROCESS BY RESPONSE SURFACE METHOD

作　　者：张志强[1,2] 高勇生[2] 袁振宏[1] 许敬亮[1] 何敏超[1] 张宇[1] 高月淑[1] 

机构地区：[1]中国科学院广州能源研究所、中国科学院可再生能源与天然气水合物重点实验室,广州510640 [2]江西农业大学国土资源与环境学院,南昌330045

出　　处：《太阳能学报》2014年第6期944-948,共5页Acta Energiae Solaris Sinica

基　　金：国家高技术研究发展(863)计划(2013AA065803);国家自然科学基金(21176237;21211140237);中科院-广西科学院院地合作项目;广州市科技攻关项目(2013J4300026)

摘　　要：利用Minitab软件对甘蔗渣酶解工艺条件进行优化研究。采用Plackett-Burman设计,从温度、pH值、加酶量、液固比和转速5个影响因素中,筛选出具有显著效应的温度、pH值和加酶量3个主要影响因素。然后采用响应面法对3个主要影响因素做进一步优化研究。优化结果表明:温度47.6℃,p H=4.76和加酶量37.76 Fpu/g时,综纤维素转化率可达74.5%。该条件下,实际试验值为74.44%,与预测值吻合较好。The Minitab Plackett-Burman desi ratio of liquid and so gn lid, software was adopted to optimize enzymatic hydrolysis process of sugarcane bagasse. Firstly, was used to evaluate five variables including temperature, pH value, cellulase adding dosage, agitation speed. The significant impact factors were screened as temperature, pH value and cellulase adding dosage. Then the response surface method （RSM） was used for further optimization study. The results showed that the optimum conditions are temperature of 47.6 ℃, pH of 4.76, cellulase adding dosage of 37.76 Fpu/g, the holocellulose conversion rate can attain to 74.5%. Under these conditions, the actual concentration of the holocellulose conversion rate is 74.44%, is in good agreement with the predicted values.

关 键 词：甘蔗渣 响应面分析 Plackett—Burman设计 酶解工艺优化 

分 类 号：Q814.9[生物学—生物工程] Q556.2