基于ITS2条形码鉴定柴胡与大叶柴胡  

Identification of Bupleurum chinense and B.longiradiatum based on ITS2 barcode

作　　者：于俊林[1,2] 赵莎[3] 任明波[4] 钱齐妮[4] 庞晓慧 

机构地区：[1]通化师范学院吉林省长白山药用植物研究重点实验室,吉林通化134002 [2]通化师范学院制药与食品科学学院,吉林通化134002 [3]中国医学科学院北京协和医学院药用植物研究所,北京100193 [4]重庆市药物种植研究所,重庆408435

出　　处：《中国中药杂志》2014年第12期2160-2163,共4页China Journal of Chinese Materia Medica

基　　金：科学技术部科技基础性工作专项(2014FY130400)

摘　　要：为准确鉴定柴胡与大叶柴胡,该研究共48份柴胡、大叶柴胡药材,扩增ITS2序列并进行测序,采用CodonCode Aligner软件进行序列拼接,用软件MEGA5.0分析比对确定不同的单倍型,并基于K2P模型进行遗传距离等分析。采用相似性搜索法、最近距离法、构建邻接树(NJ树)法对柴胡与大叶柴胡进行鉴定。结果显示柴胡与大叶柴胡种间最小K2P距离为0.049,远远大于柴胡种内最大K2P距离0.013。NJ树显示大叶柴胡独自聚为一支,可明显与柴胡区分。因此应用ITS2条形码可以准确鉴定柴胡及大叶柴胡。In this study, ITS2 barcode was used to identify Bupleurum chinense and B. longiradiatum. The ITS2 regions of 48 samples were amplified and sequenced. The sequences obtained above were aligned and the K2P distances were calculated. We used three methods, BLAST1, nearest distance and phylogenetic tree （NJ-tree）, to test the identification ability. The results showed that the maximum intraspecific genetic distance of B. chinense was 0.013, and the minimum interspecific genetic distance between B. chinense and B. longiradiatum was 0.049. The NJ-tree can easily identify B. chinense and B. longiradiatum. Therefore, the ITS2 barcode is suitable to identify B. chinense and B. longiradiatum.

关 键 词：大叶柴胡 柴胡 ITS2 DNA条形码 

分 类 号：R282.5[医药卫生—中药学]