以鹿茸对去卵巢骨质疏松症模型大鼠的影响对不同规格鹿茸进行质量评价  

Quality evaluation on different specifications of Cervi Cornu Pantotrichum with its effect on ovariectomized osteoporosis model rats

作　　者：龚伟[1,2] 李峰[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中国中药杂志》2014年第12期2326-2329,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973881)

摘　　要：目的:考察8种不同规格鹿茸对去卵巢大鼠骨质疏松症的影响,并对8种不同规格鹿茸进行等级划分。方法:雌性SD大鼠100只,随机分为10组,分别为正常组、模型组、8组不同规格鹿茸组。采用切除大鼠双侧卵巢的方法复制骨质疏松症模型,同时给予8种不同规格鹿茸,给药12周后,观察不同规格鹿茸对大鼠骨密度、血清生化指标的影响,并以不同组大鼠的骨密度,血清中ALP,BMP-2,BGP含量为影响因素,对8种不同规格鹿茸进行聚类分析。结果:给药12周后,不同规格鹿茸均能显著提高去卵巢大鼠血清中ALP,BMP-2,BGP含量,提高去卵巢大鼠的骨密度,且聚类分析结果与传统商品鹿茸药材质量划分相似。结论:不同规格鹿茸对去卵巢所致的大鼠骨质疏松症均有拮抗作用,且作用强弱与商品药材质量相关。Objective： To study the effect of eight specifications of Cervi Cornu Pantotrichum on the osteoporosis of ovariectomized rats and grade the eight different specifications of Cervi Cornu Pantotrichum. Method： Totally 100 SD female rats were divided randomly into 10 groups, namely the normal group, the model group and eight Cervi Cornu Pantotrichum groups of different specifications. Their bilateral ovaries were excised to reproduce the osteoporosis model. Meanwhile, the rats were given the eight different specifications of Cervi Cornu Pantotrichum for consecutively 12 weeks. Subsequently, the effects of the different specifications of Cervi Cornu Pantotrichum on bone mineral density and serum biochemical indicators of rats were observed. A clustering analysis was made for the eight specifications of Cervi Cornu Pantotrichum, with the serum content of ALP, BMP-2 and BGP as influencing factors. Result： After 12 weeks, the eight different specifications of Cervi Cornu Pantotrichum could significantly improve ALP, BMP-2, BGP in serum and bone mineral density of ovariectomized rats. And the cluster analysis showed similar results to the quality classification of traditional commercial herbs Cervi Cornu Pantotrichum. Conclusion： Different specifications of Cervi Cornu Pantotrichum can antagonize the osteoporosis of ovariectomized rats, and their effects are related to the quality of commercial herbs.

关 键 词：鹿茸 骨质疏松症 去卵巢大鼠 聚类分析 

分 类 号：R285.5[医药卫生—中药学]