apelin-APJ在慢性阻塞性肺疾病大鼠肺组织中的表达及其意义  

Expression of apelin-APJ in chronic obstructive pulmonary disease and its significance

作　　者：王嘉[1] 张萍[2] 李珠[1] 孙佳玮[1] 张彬[1] 孙武装[1] 

机构地区：[1]河北医科大学第一医院,050031 [2]邢台市人民医院,054001

出　　处：《实用医学杂志》2014年第12期1875-1878,共4页The Journal of Practical Medicine

基　　金：河北省卫生厅医学科学研究重点课题基金资助项目(编号:20090062)

摘　　要：目的:检测慢性阻塞性肺疾病(COPD)大鼠肺组织中apelin及其受体APJ的mRNA和蛋白表达,探讨apelin-APJ系统在COPD发生、发展中的作用。方法:烟雾暴露法制造COPD大鼠模型。实验设对照组和COPD组。COPD组大鼠每日2次在熏烟箱内烟雾暴露,每次连续被动吸烟20支(1 h),持续4个月。qRT-PCR测定大鼠肺组织中apelin mRNA及APJ mRNA表达。免疫组化法检测大鼠肺组织apelin和APJ蛋白表达。结果:COPD组apelin、APJ mRNA相对表达量显著低于对照组(P<0.01)。大鼠肺组织apelin、APJ mRNA表达与RV/(LV+S)均呈负相关(r=-0.454、-0.448,均P<0.05);与FEV0.3/FVC呈正相关(r=0.529、0.475,均P<0.05)。apelin、APJ蛋白主要表达在支气管肺组织上皮细胞、肺泡巨噬细胞、血管内皮细胞及部分肺泡壁细胞的细胞膜和细胞浆,COPD组及对照组apelin、APJ蛋白均呈阳性表达,半定量检测COPD组表达低于对照组(P<0.05)。结论:apelin及其受体APJ的mRNA和蛋白在COPD大鼠肺组织表达均下降,可能是导致大鼠COPD肺动脉高压、肺心病发生发展的重要因素。apelin-APJ系统可能成为探索防治COPD肺动脉高压、右心室肥大的新靶点。Objective To detect the expression of apelin and angiotensin I receptor related protein （APJ） in rats with chronic obstructive pulmonary disease （COPD）, and to explore the role of apelin-APJ system in the occurrence and development of COPD. Methods A rat model of COPD was established using the method of smoke exposure. SD rats were randomly divided into a control group and a COPD group. The rats in COPD group were exposed to secondhand cigarette smoke in a fume box twice a day for 4 months. Expressions of apelin mRNA and APJ mRNA in rat lung tissues were detected by real-time quantitative polymerase chain reaction （qRT-PCR）. Expressions of apelin protein and APJ protein were determined by immunohistochemistry. Results The expressions of apelin mRNA and APJ mRNA in COPD group were decreased by 44% and 13% as compared with those in the control group. The mRNA expressions of apelin and APJ in rat lung tissues were negatively correlated with RV/ （LV ＋ S） （r = -0.454 and -0.448, P 〈 0.05）, and positively related with FEV0.3/FVC （r = 0.529 and 0.475; P 〈 0.05）. Apelin and APJ were mainly expressed in the epithelial cells of the bronch and lung, alveolar macrophages, vascular endothelial cells, and the membrane and cytoplasm of some alveolar wall cells. The expression of apelin and APJ protein was lower in COPD group than in the control group （P 〈 0.05）. Conclusions The mRNA and protein expressions of apelin and APJ are decreased in the lung tissues in rats with COPD, which may be an important factor leading to the development of COPD. Apelin-APJ system might be a new target for prevention and treatment of pulmonary hypertension and right ventricular hypertrophy induced by COPD.

关 键 词：肺疾病 慢性阻塞性 APELIN APJ 实时定量聚合酶链反应 免疫组织化学 

分 类 号：R563.9[医药卫生—呼吸系统]