子宫内膜异位症不孕小鼠水通道蛋白表达及莪棱胶囊的干预作用  

Aquaporin Expression in Endometriosis Infertile Mice and Intervention Effect of Eleng Capsule

作　　者：黄绮丹[1] 黄海定[1] 吴会东[2] 黄国红[1] 廖美金[1] 邓时贵[1] 

机构地区：[1]广东省中医院,广东广州510120 [2]广东省第二人民医院,广东广州510317

出　　处：《新中医》2014年第7期196-199,共4页New Chinese Medicine

基　　金：广东省科技厅项目(编号:2009B060300015);广州中医药大学创新基金项目(编号:KAB111112K41)

摘　　要：目的:探讨子宫内膜异位症(EMTS)不孕小鼠水通道蛋白(AQP)表达的差异性及莪棱胶囊的干预作用。方法:将小鼠随机分为正常组、模型组、达那唑组、莪棱胶囊组。正常组与模型组:灌胃蒸馏水0.25 mL/10 g。达那唑组:灌胃达那唑0.4g/(kg·d)。莪棱胶囊组:灌胃莪棱胶囊4 g/(kg·d)。共30天。用直接化学发光法测定血清雌二醇水平,用RT-PCR技术测定各组子宫内膜组织AQP mRNA表达。结果:造模后小鼠雌二醇水平增高,模型组与正常组比较,差异有显著性意义(P<0.05)。达那唑组和莪棱胶囊组雌二醇接近正常组,与正常组比较,差异均无显著性意义(P>0.05)。模型组、达那唑组、莪棱胶囊组AQP1的相对表达量接近正常组,但组间比较,差异均无显著性意义(P>0.05)。模型组、达那唑组、莪棱胶囊组AQP2的相对表达量较正常组有所降低,但组间比较,差异均无显著性意义(P>0.05)。结论:子宫内膜异位症不孕小鼠血清雌激素水平增高,达那唑和莪棱胶囊均可降低雌二醇水平;子宫内膜异位症不孕小鼠水通道蛋白表达以及莪棱胶囊等的干预作用尚需进一步的研究。Objective： To investigate the aquaporin（APQ）expression in endometriosis infertile mice and the intervention effect of Eler^g capsule, Methods： The mice were divided into four groups, namely normal group, model group, Danazol group and Eleng group. The normal group and the model group were administrated with distilled water, 0.25 mL for per 10 g of body weight, and Danazol group was administrated with Danazol, 0.4 g for per kilogram of body weight, while Eleng group was administrated with Eleng capsule, 4 g for per kilogram of body weight. The treatment course lasted 30 days. The serum estradiol level was measured by direct chemiluminescence method, and the mRNA expression of aquaporins in the endometrial tissue was measured by RT-PCR technique. Results： The level of serum estradiol in the model group was higher than that in the normal group（P〈0.05）. Serum estradiol level in both Danazol group and Eleng group was close to that in the normal group（P〉 0.05）. AQP~ expression level in model group, Danazol group and Eleng group was similar to that in the normal group （P 〉0.05）, while AQP2 expression level was decreased, the inter-group difference being insignificant （P 〉0.05）. Conclusion. Endometriosis infertile mice have higher level of serum estradiol, and Danazol group and Eleng capsule have counteraction on the estradiol level. Further research is still required to explore the APQ expression and the intervention effect of Eleng capsule.

关 键 词：子宫内膜异位症(EMTS) 雌二醇 水通道蛋白(AQP) 莪棱胶囊 

分 类 号：R711.71[医药卫生—妇产科学]