中药仙鹤复方水提取物对肝癌H22细胞增殖与凋亡的影响及其作用机制  

To study the antitumor mechanism of aqueous extract of Xian-he compound

作　　者：袁学文[1] 陈毓杰[1] 张小燕[2] 黄慧青[1] 

机构地区：[1]惠州市第三人民医院药剂科,广东惠州516000 [2]惠州市河南岸医院药剂科,广东惠州516000

出　　处：《药物与人》2014年第6期150-151,共2页Medicine & People

摘　　要：目的：观察中药仙鹤复方水提取物（aqueousextract of Xian-he Compound，XHC）对肝癌H22细胞增殖与凋亡的影响度对炎症因子COX-2的影响。方法：栗用四甲基偶氮唑盐（MTT）比色法检测不同浓度的H22肝癌细胞增殖情况;Hoechst33342染色观察细胞形态学变化；western blot检测COX-2蛋白质表达；RT-PCR法检测COX-2mRNA水平表达的变化。结果：XHC各剂量组较空白对照组对肝癌H22细胞均有明显的抑制作用，以作用24h后的1．8g／mL XHC组作用最显著，抑制率为71．25％±0．32％；与空白对照组相比，LPS诱导后加1．8g／m LXHC组有较显著的凋亡率．凋亡率由1．91％±0．45％增加至8．53％±0．13％；同样作用条件下与空白对照组及阳性对照组相比COX-2蛋白质及mRNA的表达明显下调。结论：XHC的抑瘤作用与下调COX-22蛋白质及mRNA的表达有密切关系。Objeetive： To investigate the effect of XHC on the proliferation and apoptosis as well as the expression to H22 hepatoma cells in vitro. Methods： The inhibitory effect of different cone entrations of XHC on H22 cells was examined by methyl thlazolyl tetrazo lium（MTT） assay; the H22 cell apoptosis after each test is analysed by Ho- eehst 33342 staining; the expression of COX protein and mRNA was determined by western blot and RT-PCR individually. Results： each dose group of XHC compared with the control group can significant＇ty inhibit the proliferation of H22 eel.is,specially,the 1.8g/mLXHC group after 24h is the best at 71.25%±0.32%. and also, the LPS-in duced plus 1.8g/mLXHC group bad more significant apoptosis rate from 1.91%±0.45% to 8.53%± 0.13% t at this conditions, the expression of COX-2 protein and mRNA was significantly reduced compared with the control group and positive group. Conclusion：The XHC has a better anti-tumor effect in animals, which may be closely related with the COX-2.

关 键 词：中药仙鹤复方水提取物 肝癌H22 细胞株 COX-2 

分 类 号：R28[医药卫生—中药学]