美洲型和欧洲型PRRSV RT-PCR鉴别检测方法的建立  

Establishment of RT-PCR Method for Identifation Detection of PRRSV

作　　者：戴光文 谢丽华[1] 江精华[1] 李媚[1] 梁真[1] 苏志毅 严红 

机构地区：[1]梧州市动物疫病预防控制中心,广西梧州543002 [2]梧州市动物卫生监督所,广西梧州543002

出　　处：《动物医学进展》2014年第6期32-35,共4页Progress In Veterinary Medicine

基　　金：2013年广西水产畜牧业科技项目(桂渔牧科1304548);梧州市科学研究与技术开发计划(201302303)

摘　　要：在分析不同美洲型猪繁殖与呼吸综合征病毒(PRRSV)全基因组基础上,设计一对能扩增2种PRRSV毒株NSP2基因序列的特异性共有引物。RT-PCR结果显示,变异株和经典株扩增片段分别为342bp和432bp。结果表明,该方法具有快速、特异、敏感、重复性好等特点,可同时鉴别检测美洲型PRRSV变异毒株与经典毒株。临床应用于16份样品检测,PRRSV总体检出率为37.5%,高致病性特征变异株占总毒株的83.3%。In order to analyze whole genome of the different American genotype porcine reproductive and re-spiratory syndrome virus（PRRSV）,a pair of specific primer were designed to amplify two kinds of PRRSV strain NSP2 genes.RT-PCR test results indicated that the amplified fragments of mutant and classical strains were 342 bp and 432 bp.The method was rapid,specific,sensitive,repeatabe,and can be used to i-dentify mutant and classical strains of American genotype PRRSV at the same time.The clinical application in the detection of 16 samples showed that the positive rate of PRRSV was 37.5%,highly pathogenic char-acteristics of variant strains accounted for 83.3% of the total strains.

关 键 词：猪繁殖与呼吸综合征病毒 反转录-聚合酶链反应 鉴别检测方法 

分 类 号：S852.659.6[农业科学—基础兽医学]