一株羊口疮病毒分离株的生物学特性研究  

Study on Biological Characteristics of an Orf Virus Isolate

作　　者：唐娜[1] 刘吉山[1] 王玉茂[1] 王金良[1] 张倩 谢金文[1] 曲光刚[1] 沈志强[1,2] 

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东绿都生物科技有限公司,山东滨州256600

出　　处：《动物医学进展》2014年第6期49-53,共5页Progress In Veterinary Medicine

基　　金：山东省羊产业技术体系疫病防制岗位专家项目(SDAIT-09-011-07)

摘　　要：为深入开展羊口疮病毒特性研究与羊口疮的防控，通过细胞分离培养、B2L 基因序列测定及分析等试验，对从山东某羊场分离的一株羊口疮病毒分离株 SD1201进行病毒学及分子生物学特性研究。结果表明，该分离株能够引起绵羊睾丸细胞、Vero E6细胞、MDCK 细胞发生不同程度的病变，绵羊睾丸细胞病变最为明显，在18 h 内迅速发生细胞病变，病毒 TCTD50浓度为10^-4．5/mL。通过 PCR 扩增分离株 B2L基因特定片段并克隆测序，分析结果表明，该分离株与3株羊口疮病毒中国分离株亲缘关系最近，同源性为98．0％～98．2％。The biological characteristics of an isolate Orf virus isolated from a goat farm in Shandong pro-vence were investigated by cell culture,PCR amplification and B2L gene sequence analysis.The results in-dicated that the isolate SD1201 was able to propagated in sheep testicular cells,Vero E6,MDCK cells and caused cytopathogenic effect in varying degrees.The infected sheep testicular cells showed most obvious CPE 18 h after inoculation,and the TCTD50 was 10 ^-4.5/mL.The B2L gene was amplified by PCR and the sequence analysis results indicated that the homology of nucleotide sequence was between 98.0% and 98.2% as compared with three strains isolated from China.

关 键 词：羊口疮病毒 SD1201株 生物学特性 B2L基因 序列分析 

分 类 号：S858.27[农业科学—临床兽医学]