番茄SSR标记在茄子及其他茄科作物上的通用性分析  

Transferability of tomato SSR markers to eggplants and other Solanaceous vegetables

作　　者：汪国平[1] 牛玉[1,2] 汪文毅[1] 乐素菊[3] 林鉴荣 

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [3]仲恺农业工程学院生命科学学院,广东广州510225 [4]广州市农业科学研究院,广东广州510308

出　　处：《华南农业大学学报》2014年第4期56-60,共5页Journal of South China Agricultural University

基　　金：科技部国际合作项目(2010DFA32190);国家自然科学基金(31171957;30771472);广东省科技厅国际合作项目(2009B050100001;2011B050100013);广州市科技支撑计划项目(2010Z1-E381)

摘　　要：【目的】利用已完成测序的番茄基因组发展大量的SSR标记,并将这些标记转移到茄子及其他茄科作物上,节省开发SSR标记的成本.【方法】本研究利用近缘物种转移法分析了番茄SSR标记在茄子及其他茄科作物上的通用性情况.【结果和结论】1 046对番茄SSR引物中有887对能在茄子基因组DNA上扩增出产物,425对引物扩增出的带型在番茄与茄子间相似程度高,标记的通用率为40.6%;EST-SSR比基因组SSR的通用性更好,前者通用率为54.5%,后者为38.9%;414个通用SSR标记被电子定位到番茄染色体上,不同染色体来源的标记通用率明显不同;93对引物在2份用于遗传图谱构建的栽培茄子亲本间表现出多态性;获得的425对通用引物在马铃薯、辣椒、枸杞上通用率分别为96.2%、78.1%、54.1%.[Objective] Tomato genome was sequenced and a large number of SSR markers could be ob-tained .Cross-species amplification of tomato SSR markers will be an economic method to add up the number of robust SSR markers in eggplant and Solanaceous vegetables .[Method] This study tested a large set of tomato SSR markers on eggplant and other Solanaceous vegetables by close relative species transfer method .[Result and conclusion] 887 out of 1046 tested tomato SSR markers could successfully amplify on eggplants, but only 425 produced very similar bands on tomatoes and eggplants , giving a transfer rate of 40.6%.EST-SSR markers were found to be more transferable than genomic SSR markers （54.5 and 38.9%respectively ） .414 transferable SSR markers were mapped electronically onto tomato genome and their distribution on individual chromosomes highly varied .93 SSR markers showed polymor-phism on two eggplant parents used for genetic map construction .When testing 425 cross-species SSR markers on potatoes , peppers and lycium , the transferability rates were 96.2%, 78.1% and 54.1%, respectively .

关 键 词：番茄 茄子 SSR标记 通用性 茄科蔬菜 

分 类 号：S641.2[农业科学—蔬菜学]