广东和江西省柑橘溃疡病菌的遗传多样性分析  

Genetic diversities of Xanthomonas citri subsp. citri in Guangdong and Jiangxi Provinces

作　　者：魏楚丹[1] 丁钿[1] 叶淦 刘琼光[1] 

机构地区：[1]华南农业大学资源环境学院,广东广州510642 [2]江西省赣州市果业局,江西赣州341000

出　　处：《华南农业大学学报》2014年第4期71-76,共6页Journal of South China Agricultural University

基　　金：公益性行业(农业)科研专项经费项目子课题(201003067-01-7)

摘　　要：【目的】明确来自广东和江西两省柑橘溃疡病菌菌株之间的分子水平差异.【方法】使用ERIC引物,通过REP-PCR技术,对来自我国广东和江西省12个市(县)12个柑橘品种的71个柑橘溃疡病菌株进行遗传多样性研究.根据DNA指纹特征,并用NTSYS软件聚类分析.【结果和结论】71个菌株在相似值为0.64时,可以分为A、B两簇,其中来自江西赣州6个市县的所有溃疡病菌株全部聚在A簇,而来自广东有50%的菌株聚在B簇.取相似值为0.88时,A、B簇又各自分为6个遗传组.广东和江西两省柑橘溃疡病菌存在明显的遗传多样性,不同地理来源和不同柑橘品种之间的溃疡病细菌多样性差异明显.Objective] Citrus canker caused by Xanthomonas citri subsp .citri （ Xcci） is one of the most important plant diseases .The aim of the experiment was to study the genetic diversities of Xac in Guang-dong and Jiangxi Provinces .[Method] The bacterial strains which were collected from 12 cities （ coun-ties） of Guangdong and Jiangxi Provinces on 12 varieties were investigated with ERIC-PCR.Dendrograms were generated by NTSYS software according to fingerprint features .[Result and conclusion] The results indicated that 71 Xac strains could be clustered division A and B .All the strains from Jiangxi Province were distributed on division A , while half of the strains from Guangdong Province were distributed on di-vision B .Both division A and B could be seperated into 6 groups at a level of 88%similarity respective-ly.There were significant genetic diversities of Xac strains from Guangdong and Jiangxi Provinces .It showed that the diversity differences of strains from different geographical origins or different varieties were significant .

关 键 词：柑橘溃疡病 柑橘黄单胞柑橘亚种 遗传多样性 REP—PCR ERIC—PCR 

分 类 号：S666[农业科学—果树学]