柱花草胶孢炭疽菌致病力鉴定和致病类型分析  

Pathogenicity Identification of Stylo Anthracnose Pathogens and Analysis of Pathogenic Types

作　　者：易克贤[1,2] 郑金龙[1] 贺春萍[1] 习金根[1] 高建明[2] 张世清[2] 陈河龙[2] 刘巧莲[2] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室,海南海口571101 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室,海南海口571101

出　　处：《热带农业科学》2014年第5期62-68,共7页Chinese Journal of Tropical Agriculture

基　　金：国家自然科学基金"柱花草炭疽菌致病相关基因的克隆及功能分析(No.31072076)";公益性行业科研专项"草地病害防治技术研究与示范(No.201303057)"

摘　　要：用2套鉴别寄主分别对47份A型菌和57份B型菌的致病力进行鉴定,研究表明,不同的柱花草炭疽病菌株致病力不同。应用最大似然法(EML法)对鉴别寄主的致病力鉴定结果进行聚类分析,A型菌可划分成3大小种类型,且其聚类非常紧凑,表现出一定的寄生专化性;而B型菌可划分成2大小种类型,但其在型内分化较为复杂,说明B型菌将来有可能分化出更多的小种类型。从地区而言,无论A型菌还是B型菌均表现为海南菌株的致病力最强,其中尤以昌江和东方的菌株致病力最强。因而对病原变异和进化动态的监测十分必要。104 isolates （47 with biotype A,57 with biotype B） were selected for race typing based on virulence and pathogenic variation determination by using a set of host differentials. The results showed different virulence in different isolates. Total 47 of type A isolates were grouped in 3 races, and the cluster is very compact. The hosts of type A isolates were S. scabra and S. hamata in this research. And total 57 of type B in 2 races. But the differentiation within the type is relatively complex, explain the type B could differentiate into more pathogenic types in the future. As for the region, regardless of the type A or type B strains were strongest for Hainan strain pathogenicity, notably the Changjiang and Dongfang strains pathogenic strength strongest. Thus more attention should be paid to the monitoring and evaluation.

关 键 词：柱花草 炭疽病 致病力 鉴定 分析 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]