Evaluation on Inducible Effect of pOp6 Promoter in Transgenic Rice  

作　　者：Rouyi CHEN Changxiang ZHENG Jiang CHENG Minna PAN Mariena KETUDAT-CAIRNS 

机构地区：[1]Guizhou Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences [2]School of Biotechnology, Suranaree University of Technology

出　　处：《Agricultural Science & Technology》2014年第5期742-744,共3页农业科学与技术（英文版）

基　　金：Supported by Guizhou International Cooperation Project on Science and Technology[No.QiankehewaiG(2013)7040];The 20th Project of The Joint Committee on Scientific and Technical Cooperation between The Government of the Kingdom of Thailand and The Government of the People’s Republic of China(No.20-606J);China.Suranaree University of Technology grant number SUT3-304-54-12-29,Thailand

摘　　要：In order to analyze the Oslbglu4 phenotype, the inducible promoter of the transgenic rice which knock-down the Oslbglu4 expression was assessed. The result showed that 30 μM dexamethasone（DEX） had the stronger induction effect than 10 μM DEX by β-Glucuronidase （GUS） staining, qRT-PCR further verified the Oslbglu4 gene deletion. The effect of DEX and its solvent absolute ethanol on seed development was measured, and no significant effect was observed. The conclusion is that final concentration of DEX at 30 μM is suitable for pOp6 promoter induction.In order to analyze the Os1bglu4 phenotype,the inducible promoter of the transgenic rice which knock-down the Os1bglu4 expression was assessed.The result showed that 30μM dexamethasone(DEX)had the stronger induction effect than10μM DEX byβ-Glucuronidase(GUS)staining.qRT-PCR further verified the Os1bglu4 gene deletion.The effect of DEX and its solvent absolute ethanol on seed development was measured,and no significant effect was observed.The conclusion is that final concentration of DEX at 30μM is suitable for pOp6 promoter induction.

关 键 词：POp6 promoter GUS activity Inducible effect 

分 类 号：S511[农业科学—作物学] O621.12[理学—有机化学]