Determination of the Composition and Content of Lycium barbarum Polysaccharides by Chromatography  

色谱法测定宁夏枸杞多糖组成及含量(英文)

作　　者：苟春林 张艳 葛谦 

机构地区：[1]宁夏农产品质量标准与检测技术研究所,宁夏银川750002

出　　处：《Agricultural Science & Technology》2014年第5期882-884,共3页农业科学与技术（英文版）

基　　金：Supported by the Self-development Project of Ningxia Academy of Agriculture and Forestry Sciences(NKYJ-13-06)~~

摘　　要：Lycium barbarum polysaccharides （LBP） were obtained after Lycium barbarum, the study object, went through chloroform-methanol degreasing, water extraction and alcohol precipitation, acetone and ether wash and dry, hydrogen peroxide bleaching, deproteinization by savage method. Determined LBP molecular weight by HPLC was 73950-138090Da. And the monosaccharides in LBP determined by IC mainly included glucose, arabinose, rhamnose, galactose, xylose and mannose, in which glucose, arabinose and galactose had larger contents, while the contents of other monosaccharides were low.以宁夏枸杞为研究对象,经氯仿-甲醇脱脂、水提醇沉、乙醇、丙酮和乙醚洗涤干燥、双氧水脱色、savage法除蛋白后可得宁夏枸杞多糖;高效液相色谱测定枸杞多糖分子量范围为:73950-138090Da;离子色谱法测定宁夏枸杞多糖中单糖以葡萄糖、阿拉伯糖、鼠李糖、半乳糖、木糖和甘露糖为主,其中含量较多的是葡萄糖、阿拉伯糖和半乳糖,其他单糖含量较低。

关 键 词：Lycium barbarum L Lycium barbarum polysaccharides Molecular weight MONOSACCHARIDE 

分 类 号：R284[医药卫生—中药学]