何首乌3种组分的提取及其清除DPPH自由基作用的研究  

Study on separation and DPPH radical scavenging activities of 3 components from Polygonum multiflorum Thunb.

作　　者：周莹[1] 刘广锋[2] 冯娟[2] 鲁文茹 管淑玉[1] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]中国水产科学研究院南海水产研究所,广东广州510300

出　　处：《广东药学院学报》2014年第3期301-304,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金(81001702);省部产学研结合项目(2010B090400519);广东药学院人才启动基金(2007ZYX01);广东省大学生创新训练计划项目(1057312033)

摘　　要：目的分离何首乌二苯乙烯苷、蒽醌、多糖3种组分,比较它们清除DPPH自由基的能力,揭示何首乌抗氧化可能的物质基础。方法采用溶剂萃取和柱层析分离提取何首乌二苯乙烯苷、蒽醌、多糖3种组分,并通过DPPH自由基清除试验,比较各组分清除自由基的能力。结果何首乌二苯乙烯苷组分清除DPPH自由基的IC50为1.42 mg/mL(以生药计,下同);何首乌蒽醌组分清除DPPH自由基的IC50为2.67 mg/mL,何首乌多糖组分清除DPPH自由基的IC50为360 mg/mL。结论二苯乙烯苷和蒽醌是何首乌清除DPPH自由基的主要组分;何首乌多糖体外清除DPPH自由基的作用较弱。Objective To investigate the antioxidant material basis of Polygonum multiflorum Thunb.by comparing the free radical scavenging activities of stilbene glycosides,anthraquinones and polysaccharides.Methods The stilbene glycosides,anthraquinones and polysaccharides were purified by solvent extraction and column chromatographies,and their scavenging capacity of free radicals were compared by DPPH radical scavenging experiments.Results The IC50of the stilbene glycosides,anthraquinones and polysaccharides were 1.42,2.67 and 360 mg/mL（equivalent to crude drug content）,respectively.Conclusion Stilbene glycosides and anthraquinones were the main active components on DPPH radical scavenging.While polysaccharide showed weak effect.

关 键 词：DPPH自由基清除 何首乌 二苯乙烯苷 蒽醌 多糖 

分 类 号：R284.2[医药卫生—中药学]