正交试验优选养精种玉煎膏的提取工艺  

Optimization of the extraction process of Yangjing Zhongyu decoction

作　　者：李龙[1] 潘巧波 王岩[1] 麦泳瑶 翁艺芳 王婴[3] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]中山市横栏医院,广东中山528478 [3]广东药学院医药化工学院,广东中山528458

出　　处：《广东药学院学报》2014年第3期305-308,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省中医药局管理科研基金(20131253)

摘　　要：目的优选养精种玉煎膏的提取工艺。方法采用正交试验法,考察加水量、提取时间、提取次数3个因素对提取工艺的影响,以芍药苷、马钱苷质量分数及浸膏得率为综合评价指标;采用RP-HPLC法测定芍药苷、马钱苷质量分数,烘干法测定浸膏得率。结果最佳提取工艺为药材加6倍量水,加热回流提取3次,每次提取时间为1 h。结论该工艺设计合理、稳定可行,适用于工业化生产。Objective To optimize the extraction process of Yangjing Zhongyu decoction.Methods Orthogonal design was used to investigate the amount of water,extraction times and extraction time with the yields of paeoniflorin,loganin and extract yield as indicators.The concentration of paeoniflorin and loganin were determined by RP-HPLC,and the extract yield was determined by drying method.Results The best extraction conditions were as follows： refluxing 3 times with 6-fold water and 1 h for each time.Conclusion This optimized extraction process was legitimate,stable,feasible,and applicable for industrial production.

关 键 词：养精种玉煎膏 芍药苷 马钱苷 正交试验 

分 类 号：TQ461[化学工程—制药化工]