超高效液相与质谱联用同时检测胆汁酸含量  

Simultaneous detection of multiple bile acids content using ultra-fast liquid chromatography-mass spectrometry technology

作　　者：喻振文 高欣[2] 房中则[3] 曹云峰[3] 孙晓宇[3] 洪沫 王东[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]军事医学科学院附属医院临床药理实验室,北京100071 [3]辽宁医学院附属第一医院转化医学中心,辽宁锦州121000

出　　处：《转化医学电子杂志》2014年第3期129-132,共4页E-Journal of Translational Medicine

摘　　要：目的：通过建立快速高效的UFLC—MS／MS方法同时检测分析胆汁酸类化合物，以便于更好地定量分析胆汁酸的在体水平．方法：以甘草次酸为内标，采用超高效液相与质谱联用技术同时测定13个胆汁酸类化合物，并评价该方法的精密度，准确度，及线性范围．结果：建立了能同时检测以甘草次酸为内标13个胆汁酸化合物的UFLC／MS／MS检测方法，各成分分离度好，13种胆汁酸的浓度在1～20000nmol／L的浓度范围有良好的线性关系；r在0．995—0．999之间；检出限为0．065—9．37nmol／L；方法的日内和日间准确度在97％-105％之间，日间日内精密度均小于8％．结论：建立的UFLC／MS／MS方法灵敏度高，特异性好，适用于胆汁酸体外的分析研究．AIM： To quantitative analyze the level of bile acids in vivo, a rapid and efficient UFLC-MS/MS analysis method was performed to detect multiple bile acids. METHODS： Compound glycyrrhetinic acid was used as the internal standard （IS）. Ultrafast liquid chromatography-mass spectrometry technology was applied to simultaneously detect 13 bile acids compounds. Also the accuracy, precision and the linear range of the method was assessed. RESULTS： An UFLC/MS/MS analysis method which could respectively detect 13 bile acids and IS glycyrrhetinic was established. All 13 bile acids compounds were obtained well linear relationship with concentration range of 1 - 20000 nmol/L. The limit of detection （LOD） were defined 0. 065 - 9.37 nmol/L. The intra- and inter-day accuracy ranged from 97% - 105%, and the intra- and inter-day precision were all less than 8%. CONCLUSION： The UFLC/MS/MS method with good sensitivity and selectivity could be applied to investigate bile acids in vitro.

关 键 词：胆汁酸 超高效液相与质谱联用 分析方法开发 

分 类 号：R917[医药卫生—药物分析学]