骨髓间充质干细胞移植在糖尿病小鼠创面血管生成及愈合中的作用  

Role of bone marrow mesenchymal stem cell transplantation in angiogenesis and wound healing of diabetic mice

作　　者：谷城威[1,2] 马奎[2] 高冬蕴[1,2] 沈涛[1] 付小兵[2] 吴旭[1] 

机构地区：[1]南方医科大学附属南方医院,广州510515 [2]解放军总医院第一附属医院,全军创伤修复与组织再生重点实验室

出　　处：《山东医药》2014年第21期22-25,共4页Shandong Medical Journal

基　　金：国家重点基础研究发展计划(973计划)(2012CB518105)

摘　　要：目的探讨骨髓间充质干细胞(BMMSCs)局部移植对糖尿病(DM)小鼠创面血管生成及愈合中的作用。方法采用链脲佐菌素诱导C57BL/6小鼠构建T2DM模型,取其BMMSCs培养至P3代,采用流式细胞技术检测细胞表面抗原Sca-1、CD14、CD29、CD34、CD45、CD90、CD105表达,以及细胞的克隆形成和成骨、成脂能力;以正常小鼠P3代BMMSCs为对照。将80只T2DM建模小鼠随机均分为正常组、DM组、胎牛血清(FBS)组及空白组,均于左下肢构建6 mm创面;实验0、2 d取正常组、DM组的创面组织冰冻切片示踪其分布情况。记录0、7、14 d各组创面愈合情况,并对其7、14 d组织行血管内皮生长因子(VEGF)检测。结果与正常组相比,DM组的CD29、CD90阳性表达率降低(P均<0.01),Sca-1、CD105阳性表达率两组比较P>0.05;两组均阴性表达CD14、CD34、CD45。DM组的克隆形成及分化能力较正常组降低。正常组、DM组创面愈合率均优于对照组和PBS组(P均<0.05),其中正常组优于DM组。正常组创面VEGF表达水平高于其他各组(P均<0.05),而DM组略高于PBS组和空白组。结论BMMSCs局部移植可增加DM小鼠创面血管生成,从而促进其愈合,但DM组的作用弱于正常组,可能与其生物学性能受影响有关。Objective To investigate the effect of bone marrow mesenchymal stem cells （BMMSCs） local transplanta-tion on the angiogenesis and wound healing of mice with diabetes mellitus （DM）.Methods C57BL/6 mice were induced as a T2DM model by streptozotocin , and BMMSCs from diabetic mice were cultured routinely to the P 3 generation.Flow cy-tometry was used to determine the expression levels of cell surface antigens including Sca -1, CD14 , CD29 , CD34 , CD45 , CD90 and CD105 .And the capacities of colony forming and osteogenesis and adipogenesis differentiation were also detected . BMMSCs from the P3 generation of normal mice were also cultured as controls .Eighty T2DM model mice were randomly di-vided into four groups including the normal group , DM group, fetal bovine serum （PBS） group and the control group, and 6 mm wounds were constructed in the left extremity .Frozen sections of wound tissues from the normal group and DM group were done to trace its distribution at day 0 and 2.Generally healing of the wounds was recorded respectively at day 0, 7 and 14, and the expression of vascular endothelial growth factor （VEGF） in the wound tissues at day 7 and 14 was detected. Results Compared with the normal group , the positive expression rate of CD 29 and CD90 was both significantly lower in the DM group （all P＆lt;0.01）, and there was no significant difference between the two groups in the positive expression rate of Sca-1and CD105（P＆gt;0.05）, meanwhile, negative expression of CD14, CD34 and CD45 was detected in both groups.The colony forming and differentiation capacity in the DM group were also weaker than that of the normal group .Wound healing rates in the normal and DM groups were both superior to those of the control group and the PBS group （all P〈0.05）, and the effect in the normal group was better than that in the DM group .The expression of VEGF in the normal group was sig-nificantly higher than that of other groups （all P〉0.05）, while DM group was only slightly higher than t

关 键 词：糖尿病 骨髓间充干细胞 血管内皮生长因子 创面愈合 小鼠 

分 类 号：R587.1[医药卫生—内分泌]