Glucose detection at attomole levels using dynamic light scattering and gold nanoparticles  

作　　者：MIAO XiangMin FENG ZhaoZhong TIAN Jun PENG Xue 

机构地区：[1]School of Life Science, Jiangsu Normal University

出　　处：《Science China Chemistry》2014年第7期1026-1031,共6页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21305053);the Natural Science Fund for Colleges and Universities in Jiangsu Province(13KJB150015);the Natural Science Fund in Jiangsu Province(BK20130227);the Scientific Research Support Project for Teachers with Doctor’s Degrees(Jiangsu Normal University,China,12XLR022);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘　　要：Glucose is directly related to brain activity and to diabetes. Therefore, developing a rapid and sensitive method for glucose de- tection is essential. Here, label-free glucose detection at attomole levels was realized by detecting the average diameter change of gold nanoparticles （AuNPs） utilizing dynamic light scattering （DLS）. Single-strand DNA （ssDNA） adsorbed into the AuNPs＇ surfaces and prevented them from aggregating in solution that contained NaC1. However, ssDNA cleaved onto ssDNA fragments upon addition of glucose, and these fragments could not adsorb onto the AuNPs＇ surfaces. Therefore, in high-salt solution, AuNPs would aggregate and their average diameter would increase. Based on monitoring the average diameter of AuNPs with DLS, glucose could be detected in the range from 15 pmol/L to 2.0 nmol/L, with a detection limit of 8.3 pmol/L. Satisfactory results were also obtained when the proposed method was applied in human serum glucose detection.Glucose is directly related to brain activity and to diabetes.Therefore,developing a rapid and sensitive method for glucose detection is essential.Here,label-free glucose detection at attomole levels was realized by detecting the average diameter change of gold nanoparticles(AuNPs)utilizing dynamic light scattering(DLS).Single-strand DNA(ssDNA)adsorbed into the AuNPs’surfaces and prevented them from aggregating in solution that contained NaCl.However,ssDNA cleaved onto ssDNA fragments upon addition of glucose,and these fragments could not adsorb onto the AuNPs’surfaces.Therefore,in high-salt solution,AuNPs would aggregate and their average diameter would increase.Based on monitoring the average diameter of AuNPs with DLS,glucose could be detected in the range from 15 pmol/L to 2.0 nmol/L,with a detection limit of 8.3 pmol/L.Satisfactory results were also obtained when the proposed method was applied in human serum glucose detection.

关 键 词：GLUCOSE dynamic light scattering gold nanoparticles 

分 类 号：O629.11[理学—有机化学] TQ131.23[理学—化学]