羊水染色体制备技术改良的研究  

Studies on the techniques improving of amniotic fluid chromosome preparation

作　　者：张玲莉[1] 陈小波[1] 严庆庆 朱庆文[1] 朱自力[2] 

机构地区：[1]南通市妇幼保健院产前筛查与诊断中心 [2]南通市肿瘤医院检验科,江苏南通226000

出　　处：《中国优生与遗传杂志》2014年第6期41-42,47,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨染色体制备的技术改良,提高羊水细胞染色体制备的成功率和工作效率。方法采用染色体改良技术(改良法)与传统的胰酶制备技术(胰酶法)进行比较分析。结果改良法所获得的可供分析的染色体数目为6.91±1.711,胰酶法所获得的可供分析的染色体数目为5.37±1.324,t=5.861,P<0.001。结论改良法所制备的染色体的核型数与可供分析的染色体数目均多于胰酶法,而且操作简单、能提高工作效率,值得推广应用。Objective： To investigate the improvement of technology of amniotic fluid chromosome preparation, improve the success rate and definition of chromosome preparation. Methods： Improved technology and traditional chromosome trypsin preparation techniques were compared and analyzed. Results： The number of chromosomes available for analysis obtained from improved method and traditional techniques was 6.91±1.711 and 5.37±1.324, respectively （t=5.861, P〈0.001） . Conclusion： Improved chromosome preparation techniques should be widely applied for high achievement rate and zonation of karyotype, as well as ease of operation.

关 键 词：羊水细胞 染色体制备 胰酶法 改良法 

分 类 号：R714.5[医药卫生—妇产科学]