姜黄素对人牙髓细胞周期及凋亡的影响  

The role of curcumin in the cell cycle and apoptosis of HDPCs

作　　者：刘惠娟[1] 徐琼[1] 

机构地区：[1]中山大学光华口腔医学院.广东省口腔医学重点实验室,硕士生广东510055

出　　处：《中华老年口腔医学杂志》2014年第2期65-69,共5页Chinese Journal of Geriatric Dentistry

基　　金：国家自然科学基金项目(项目编号:81170953)

摘　　要：目的:探讨姜黄素对人牙髓细胞(human dental pulp cells,HDPCs)细胞周期及凋亡的影响。方法:体外培养人健康牙髓细胞,不同浓度姜黄素(curcumin)处理细胞后流式细胞术检测细胞周期,TUNEL染色法观察细胞凋亡,实时荧光定量PCR及免疫蛋白印迹分别检测p300 mRNA及蛋白水平。结果:流式细胞术及TUNEL结果显示姜黄素处理人牙髓细胞后,G0/G1期细胞比例上升,S期细胞比例下降,凋亡细胞增加;实时荧光定量PCR及免疫蛋白印迹结果显示姜黄素抑制p300 mRNA及蛋白的表达。结论:姜黄素使细胞周期停滞在G0/G1期,诱导牙髓细胞凋亡,其机制可能是通过下调p300活性。Objectives： The purpose of this study was to investigate the role ofcurcumin in the cell cycle and apoptosis of HDPCs. Methods： The cell cycle and apoptosis of the HDPCs were analyzed using flow cytometry and TUNEL staining, respectively. Real time-qPCR and western blotting analysis were utilized to analyze the mRNA and protein expression of p300 after curcumin impacted the HDPCs. Results： Curcumin arrested cells in the G0/G1 phase and triggered apoptosis of HDPCs. p300 was down-regulated at the mRNA and protein levels by curcumin. Conclusion： Curcumin affected the the cell cycle and apoptosis of HDPCs by down-regulation of p300.

关 键 词：姜黄素 P300 牙髓细胞 细胞周期 细胞凋亡 

分 类 号：R285[医药卫生—中药学]